Heterochromatin protein 1 (HP1) connects the FACT histone chaperone complex to the phosphorylated CTD of RNA polymerase II

被引:76
作者
Kwon, So Hee [1 ]
Florens, Laurence [1 ]
Swanson, Selene K. [1 ]
washburn, Michael P. [1 ,2 ]
Abmayr, Susan M. [1 ,3 ]
Workman, Jerry L. [1 ]
机构
[1] Stowers Inst Med Res, Kansas City, MO 64110 USA
[2] Univ Kansas, Dept Pathol & Lab Med, Med Ctr, Kansas City, KS 66160 USA
[3] Univ Kansas, Dept Anat & Cell Biol, Med Ctr, Kansas City, KS 66160 USA
关键词
HP1c; FACT; transcription elongation; heat-shock genes; HSP70; TRANSCRIPTION ELONGATION-FACTORS; NONHISTONE CHROMOSOMAL PROTEIN; GENE-EXPRESSION; IN-VIVO; SACCHAROMYCES-CEREVISIAE; DROSOPHILA-MELANOGASTER; CHROMATIN-STRUCTURE; PAF1; COMPLEX; HEAT-SHOCK; YEAST;
D O I
10.1101/gad.1959110
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Heterochromatin protein 1 (HP1) is well known as a silencing protein found at pericentric heterochromatin. Most eukaryotes have at least three isoforms of HP1 that play differential roles in heterochromatin and euchromatin. In addition to its role in heterochromatin, HP1 proteins have been shown to function in transcription elongation. To gain insights into the transcription functions of HP1, we sought to identify novel HP1-interacting proteins. Biochemical and proteomic approaches revealed that HP1 interacts with the histone chaperone complex FACT (facilitates chromatin transcription). HP1c interacts with the SSRP1 (structure-specific recognition protein 1) subunit and the intact FACT complex. Moreover, HP1c guides the recruitment of FACT to active genes and links FACT to active forms of RNA polymerase II. The absence of HP1c partially impairs the recruitment of FACT into heat-shock loci and causes a defect in heat-shock gene expression. Thus, HP1c functions to recruit the FACT complex to RNA polymerase II.
引用
收藏
页码:2133 / 2145
页数:13
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