A nontoxic, photostable and high signal-to-noise ratio mitochondrial probe with mitochondrial membrane potential and viscosity detectivity

被引：16
作者：
Chen, Yanan [2 ]
Qi, Jianguo [1 ]
Huang, Jing [1 ]
Zhou, Xiaomin [1 ]
Niu, Linqiang [1 ]
Yan, Zhijie [1 ]
Wang, Jianhong [1 ]
机构：
[1] Henan Univ, Key Lab Nat Med & Immunoengn Henan Prov, Jinming Campus, Kaifeng 475004, Henan, Peoples R China
[2] Henan Univ, Inst Behav & Psychol, Jinming Campus, Kaifeng 475004, Henan, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2018年 / 189卷
关键词：
Fluorescent probe; Mitochondria-targeting; Mitochondrial membrane potential; Viscosity; TARGETABLE FLUORESCENT SENSOR; TARGETING MITOCHONDRIA; LIVING CELLS; ZINC IONS; PEROXYNITRITE; DEATH; PH;
D O I：
10.1016/j.saa.2017.08.063
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Herein, we reported a yellow emission probe 1-methyl-4-(6-morpholino-1, 3-dioxo-1H-benzol[de]isoquinolin-2(3H)-yl) pyridin-1-ium iodide which could specifically stain mitochondria in living immortalized and normal cells. In comparison to the common mitochondria tracker (Mitotracker Deep Red, MTDR), this probe was nontoxic, photostable and ultrahigh signal-to-noise ratio, which could real-time monitor mitochondria for a long time. Moreover, this probe also showed high sensitivity towards mitochondria( membrane potential and intramitochondrial viscosity change. Consequently, this probe was used for imaging mitochondria, detecting changes in mitochondrial membrane potential and intramitochondrial viscosity in physiological and pathological processes. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：634 / 641
页数：8
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