Simplified approach for quantitative digital holographic phase contrast imaging of living cells

被引：182

作者：

Kemper, Bjoern ^{[1
]}

Vollmer, Angelika ^{[1
]}

Rommel, Christina E. ^{[2
]}

Schnekenburger, Juergen ^{[2
]}

von Bally, Gert ^{[1
]}

机构：

[1] Univ Munster, Ctr Biomed Opt & Photon, D-48149 Munster, Germany

[2] Univ Munster, Dept Med B, D-48149 Munster, Germany

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2011年 / 16卷 / 02期

关键词：

quantitative phase contrast; digital holographic microscopy; live cell imaging; DIFFRACTION TOMOGRAPHY; MICROSCOPY; OPTIMIZATION;

D O I：

10.1117/1.3540674

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Many interferometry-based quantitative phase contrast imaging techniques require a separately generated coherent reference wave. This results in a low phase stability and the demand for a precise adjustment of the intensity ratio between object and reference wave. To overcome these problems, the performance of a Michelson interferometer approach for digital holographic microscopy was analyzed that avoids a separately generated reference wave by superposition of different image areas. It is shown that this simplified arrangement yields improved phase stability. Furthermore, results from time-lapse investigations on living pancreas tumor cells demonstrate the capability of the method for reliable quantitative phase contrast imaging. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3540674]

引用

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