Maternal Dead-end 1 promotes translation of nanos1 by binding the eIF3 complex

被引:30
作者
Aguero, Tristan [1 ]
Jin, Zhigang [2 ]
Chorghade, Sandip [3 ]
Kalsotra, Auinash [3 ]
King, Mary Lou [1 ]
Yang, Jing [2 ]
机构
[1] Univ Miami, Dept Cell Biol, Miami, FL 33136 USA
[2] Univ Illinois, Dept Comparat Biosci, Urbana, IL 61802 USA
[3] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA
来源
DEVELOPMENT | 2017年 / 144卷 / 20期
基金
美国国家卫生研究院;
关键词
Germline development; Translation regulation; Dnd1; Nanos; Xenopus; GERM-CELL DEVELOPMENT; HUNCHBACK MESSENGER-RNA; DROSOPHILA EMBRYOS; PLASM COMPONENT; XENOPUS-LAEVIS; MITOCHONDRIAL CLOUD; GENE-EXPRESSION; PUMILIO; PROTEIN; INITIATION;
D O I
10.1242/dev.152611
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In the developing embryo, primordial germ cells (PGCs) represent the exclusive progenitors of the gametes, and their loss results in adult infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must transiently silence their genome, an early developmental process that requires Nanos activity. However, it is unclear how Nanos translation is regulated in developing embryos. We report here that translation of nanos1 after fertilization requires Dead-end 1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, expression of which is low in oocytes, but increases dramatically after fertilization, directly interacts with, and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development.
引用
收藏
页码:3755 / 3765
页数:11
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