Measuring of Binding kinetics of biomolecular interactions Using a Localized Surface Plasmon Couple Fluorescence Fiber-Optic Biosensor

In this study, we describe a novel method for analyzing protein-protein binding kinetics at ultra-low concentration (1 pg/mL) using a localized surface plasmon coupled fluorescence fiber-optic biosensor (LSPCF-FOB). The association and dissociation rate constants, ka and kd, respectively, for the binding kinetics of the mouse IgG/ anti-mouse IgG interaction have been calculated to be ka = (5.9928 +/- 3.1540) x 10(6) M(-1)s(-1) and kd = (1.0587 +/- 0.5572) x 10(-3) s(-1). The theoretical basis of this analytical approach is a rapid-mixing model integrated with a two-compartment model; has been experimentally verified in this study as well. The LSPCF-FOB provides a potentially alternative option for characterizing the interaction of biomolecules at ultra-low concentrations.