Retention and Separation Efficiency of Some Synthetic Oligopeptides in Reversed-Phase Thin-Layer Chromatography

被引：11

作者：

Gwarda, R. L. ^{[1
]}

Tomczyszyn, A. ^{[2
]}

Misicka, A. ^{[2
]}

Dzido, T. H. ^{[1
]}

机构：

[1] Med Univ Lublin, Dept Phys Chem, Chair Chem, PL-20093 Lublin, Poland

[2] Univ Warsaw, Fac Chem, PL-02093 Warsaw, Poland

来源：

ACTA CHROMATOGRAPHICA | 2015年 / 27卷 / 01期

关键词：

peptide retention; peptide separation; reversed-phase chromatography; thin-layer chromatography; TRYPTIC PROTEIN DIGESTS; IONIZATION MASS-SPECTROMETRY; HPTLC/DESI-MS; PEPTIDES; SENSITIVITY;

D O I：

10.1556/AChrom.27.2015.1.1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Influence of the following variables such as adsorbent type, type and concentration of organic modifier in mobile phase, type and concentration of ion-pairing reagent, or pH of the mobile phase buffer on retention of some synthetic peptides in reversed-phase high-performance thin-layer chromatography systems has been investigated. The investigations have been also focused on influence of the variables mentioned on solute zone shape regarding optimization of their separation. Remarks about solute retention mechanism have been also provided.

引用

页码：1 / 14

页数：14

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