Determination of soluble CD44 in serum by using a label-free aptamer based electrochemical impedance biosensor

被引：30

作者：

Zhou, Jie ^{[1
]}

Cheng, Kai ^{[1
]}

Chen, Xuan ^{[2
]}

Yang, Rui ^{[1
]}

Lu, Mudan ^{[1
]}

Ming, Lan ^{[1
]}

Chen, Yu ^{[1
]}

Lin, Zhenyu ^{[3
]}

Chen, Daozhen ^{[1
]}

机构：

[1] Nanjing Med Univ, Affiliated Wuxi Matern & Child Hlth Care Hosp, Dept Cent Lab, Wuxi 214002, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Kangda Coll, Lianyungang 222000, Jiangsu, Peoples R China

[3] Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Analyt Sci Food Safety & Biol, Minist Educ,Coll Chem, Fuzhou 350116, Fujian, Peoples R China

来源：

ANALYST | 2020年 / 145卷 / 02期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

LINKED-IMMUNOSORBENT-ASSAY; PROGNOSTIC VALUE; CANCER; CELLS; APTASENSOR; RECEPTORS; SELECTION; SENSOR; ACID;

D O I：

10.1039/c9an01764j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

CD44 is a promising biomarker in the diagnosis and prognosis of malignancies. The serum CD44 level is closely related to disease progression and metastasis of malignancies. It is of great clinical significance for the detection of serum soluble CD44. In this study, a facile, label-free aptamer based electrochemical impedance sensor for serum CD44 has been proposed. The aptamer showing high affinity to CD44 was immobilized on the gold electrodes through Au-S interaction. The interaction between target CD44 and the immobilized aptamer will cause a complex structure change of the aptamer, which makes the diffusion of [Fe(CN)(6)](3-/4-) toward the electrode surface easy, thus resulting in the decrease of the impedance of the system. The decreased degree of the impedance had a good linear relationship with the logarithm of the CD44 concentration in the range of 0.1-1000 ng mL(-1) with a detection limit of 0.087 ng mL(-1) (S/N = 3). The developed biosensor has been applied to detect CD44 in serum samples with satisfactory results.

引用

页码：460 / 465

页数：6

共 29 条

[1] The role of albumin receptors in regulation of albumin homeostasis: Implications for drug delivery [J].