Human antigen-presenting cells respond differently to gut-derived probiotic bacteria but mediate similar strain-dependent NK and T cell activation

被引:39
作者
Fink, Lisbeth N.
Zeuthen, Louise H.
Ferlazzo, Guido
Frokiaer, Hanne
机构
[1] Tech Univ Denmark, Ctr Biol Sequence Anal, Lyngby, Denmark
[2] Univ Messina, Dept Human Pathol, Messina, Italy
来源
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2007年 / 51卷 / 03期
关键词
antigen-presenting cell; intestinal bacteria; effector cell function; dendritic cell; monocyte;
D O I
10.1111/j.1574-695X.2007.00333.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The intestinal microbiota is essential for homeostasis of the local and systemic immune system, and particularly strains of lactic acid bacteria and Escherichia coli have been shown to have balancing effects on inflammatory conditions such as allergy and inflammatory bowel disease. However, in vitro assessment of the immunomodulatory effects of distinct strains may depend strongly on the cell type used as a model. To select the most appropriate model for screening of beneficial bacteria in human cells, the response to strains of intestinal bacteria of three types of antigen-presenting cells (APC) was compared; blood myeloid dendritic cells (DC), monocyte-derived DC and monocytes, and the effector response of natural killer cells and naive T cells was characterized. Maturation induced by gut-derived bacteria differed between APC, with blood DC and monocytes responding with the production of IL-6 and tumour necrosis factor-alpha to bacteria, which elicited mainly IL-10 in monocyte-derived DC. In contrast, comparable IFN-gamma production patterns were found in both natural killer cells and T cells induced by all bacteria-matured APC. An inhibitory effect of certain strains on this IFN-gamma production was also mediated by all types of APC. The most potent responses were induced by monocyte-derived DC, which thus constitute a sensitive screening model.
引用
收藏
页码:535 / 546
页数:12
相关论文
共 37 条
  • [31] CpG and double-stranded RNA trigger human NK cells by Toll-like receptors: Induction of cytokine release and cytotoxicity against tumors and dendritic cells
    Sivori, S
    Falco, M
    Della Chiesa, M
    Carlomagno, S
    Vitale, M
    Moretta, L
    Moretta, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (27) : 10116 - 10121
  • [32] Selective probiotic bacteria induce IL-10-producing regulatory T cells in vitro by modulating dendritic cell function through dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin
    Smits, HH
    Engering, A
    van der Kleij, D
    de Jong, EC
    Schipper, K
    van Capel, TMM
    Zaat, BAJ
    Yazdanbakhsh, M
    Wierenga, EA
    van Kooyk, Y
    Kapsenberg, ML
    [J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2005, 115 (06) : 1260 - 1267
  • [33] THOMAS R, 1993, J IMMUNOL, V151, P6840
  • [34] Dendritic cells in germ-free and specific pathogen-free mice have similar phenotypes and in vitro antigen presenting function
    Walton, KLW
    He, JP
    Kelsall, BL
    Sartor, RB
    Fisher, NC
    [J]. IMMUNOLOGY LETTERS, 2006, 102 (01) : 16 - 24
  • [35] The intestinal bacterial colonisation in preterm infants: A review of the literature
    Westerbeek, Elisabeth A. M.
    van den Berg, Anemone
    Lafeber, Harrie N.
    Knol, Jan
    Fetter, Willem P. F.
    van Elburg, Ruurd M.
    [J]. CLINICAL NUTRITION, 2006, 25 (03) : 361 - 368
  • [36] Natural killer cells and Helicobacter pylori infection:: Bacterial antigens and interleukin-12 act synergistically to induce gamma interferon production
    Yun, CH
    Lundgren, A
    Azem, J
    Sjöling, Å
    Holmgren, J
    Svennerholm, AM
    Lundin, BS
    [J]. INFECTION AND IMMUNITY, 2005, 73 (03) : 1482 - 1490
  • [37] Lactic acid bacteria inducing a weak interleukin-12 and tumor necrosis factor alpha response in human dendritic cells inhibit strongly stimulating lactic acid bacteria but act synergistically with gram-negative bacteria
    Zeuthen, LH
    Christensen, HR
    Frokiær, H
    [J]. CLINICAL AND VACCINE IMMUNOLOGY, 2006, 13 (03) : 365 - 375