Follistatin production by skin fibroblasts and its regulation by dexamethasone

被引:14
作者
Kawakami, S
Fujii, Y
Winters, SJ
机构
[1] Univ Louisville, Div Endocrinol & Metab, Sch Med, Louisville, KY 40202 USA
[2] Univ Pittsburgh, Dept Med, Pittsburgh, PA 15213 USA
关键词
follistatin; glucocorticoids; fibroblasts;
D O I
10.1016/S0303-7207(00)00371-3
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Activin and follistatin (FS) appear to play a role in the development of the skin and its appendages, in the inflammatory process, angiogenesis, and in wound healing. Although there is information on the expression of activin subunits and receptors in fibroblasts and keratinocytes, there are no reports on the regulation of FS expression in these cells. In the present study we analyzed the splicing variants of FS mRNAs in fibroblasts from genital and nongenital skin by RT-PCR and northern analysis, and examined the induction of FS mRNA and protein by hormones and growth factors in skin fibroblasts from human and nonhuman primates. FS mRNA was highly expressed in all fibroblast strains with similar expression regardless of donor species (human or monkey), donor age (neonate or adult), or the organ from which the fibroblast strains were established (skin or pituitary, genital or non-genital skin). Moreover, the band density corresponding to FS-288 was < 5-10% of the value for FS-315 in skin fibroblasts as in all other tissues examined. Fibroblast FS mRNA and protein production were biphasically regulated by dexamethasone: low concentrations (0.01 and 0.1 nM) increased whereas higher concentrations (> 1 nM) suppressed FS expression. On the other hand, androgens, activin and PACAP38 were without effect. These data establish cultured skin fibroblasts as a model to study FS gene expression in humans, and support a role for follistatin in the normal immune response and in the anti-inflammatory actions of glucocorticoids. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:157 / 167
页数:11
相关论文
共 48 条
[21]   Plasma follistatin concentrations increase following lipopolysaccharide administration in sheep [J].
Klein, R ;
Clarke, IJ ;
Hedger, MP ;
Robertson, DM .
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 1996, 23 (08) :754-755
[22]   RADIOIMMUNOASSAY OF FSH-SUPPRESSING PROTEIN IN THE EWE - CONCENTRATIONS DURING THE ESTROUS-CYCLE AND FOLLOWING OVARIECTOMY [J].
KLEIN, R ;
FINDLAY, JK ;
CLARKE, IJ ;
DEKRETSER, DM ;
ROBERTSON, DM .
JOURNAL OF ENDOCRINOLOGY, 1993, 137 (03) :433-443
[23]   PITUITARY ADENYLATE-CYCLASE POLYPEPTIDE (PACAP) STIMULATES CYCLIC-AMP FORMATION IN PITUITARY FIBROBLASTS AND 3T3 TUMOR FIBROBLASTS - LACK OF ENHANCEMENT BY PROTEIN-KINASE-C ACTIVATION [J].
KOCH, B ;
LUTZBUCHER, B .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1992, 87 (1-3) :79-86
[24]  
Kozian DH, 1997, LAB INVEST, V76, P267
[25]  
Leech M, 1999, ARTHRITIS RHEUM, V42, P1601, DOI 10.1002/1529-0131(199908)42:8<1601::AID-ANR6>3.0.CO
[26]  
2-B
[27]  
Mather JP, 1997, P SOC EXP BIOL MED, V215, P209
[28]   FUNCTIONAL-ANALYSIS OF ACTIVINS DURING MAMMALIAN DEVELOPMENT [J].
MATZUK, MM ;
KUMAR, TR ;
VASSALLI, A ;
BICKENBACH, JR ;
ROOP, DR ;
JAENISCH, R ;
BRADLEY, A .
NATURE, 1995, 374 (6520) :354-356
[29]   MULTIPLE DEFECTS AND PERINATAL DEATH IN MICE DEFICIENT IN FOLLISTATIN [J].
MATZUK, MM ;
LU, NF ;
VOGEL, H ;
SELLHEYER, K ;
ROOP, DR ;
BRADLEY, A .
NATURE, 1995, 374 (6520) :360-363
[30]   FOLLISTATINS - MORE THAN FOLLICLE-STIMULATING-HORMONE SUPPRESSING PROTEINS [J].
MICHEL, U ;
FARNWORTH, P ;
FINDLAY, JK .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1993, 91 (1-2) :1-11