Inhibition of polyphenol oxidase by copper-metallothionein from Aspergillus niger

被引：31

作者：

Goetghebeur, M ^{[1
]}

Kermasha, S ^{[1
]}

机构：

[1] MCGILL UNIV, DEPT FOOD SCI & AGR CHEM, ST ANNE DE BELLEVUE, PQ H9X 3V9, CANADA

来源：

PHYTOCHEMISTRY | 1996年 / 42卷 / 04期

关键词：

Aspergillus niger lingus; metallothionein; polyphenol oxidase; inhibition;

D O I：

10.1016/0031-9422(96)86993-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metallothionein from Aspergillus niger was found to be an inhibitor, in a model system, for the enzymic activity of a commercially purified mushroom tyrosinase. The inhibitory effect of metallothionein was higher on catechin oxidation compared with that on chlorogenic acid. The degree of inhibition of enzymic colour formation by metallothionein, using catechin as substrate; was lower than that of oxygen uptake; however, a reverse pattern was observed with chlorogenic acid as substrate. The demetallization of metallothionein did not increase its inhibitory effect on polyphenol oxidase whereas pre-incubation of the enzyme and metallothionein increased this inhibitory effect.

引用

页码：935 / 940

页数：6

共 28 条

[1]

BREMNER I, 1991, METHOD ENZYMOL, V205, P25

[2]

BYRD J, 1988, J BIOL CHEM, V263, P6688

[3] L-MIMOSINE, A SLOW-BINDING INHIBITOR OF MUSHROOM TYROSINASE [J].

CABANES, J ;

GARCIACANOVAS, F ;

TUDELA, J ;

LOZANO, JA ;

GARCIACARMONA, F .

PHYTOCHEMISTRY, 1987, 26 (04) :917-919

[4] CYSTEINE AS AN INHIBITOR OF POLYPHENOL OXIDASE [J].

DUDLEY, ED ;

HOTCHKISS, JH .

JOURNAL OF FOOD BIOCHEMISTRY, 1989, 13 (01) :65-75

[5] MECHANISM OF SULFITE INHIBITION OF BROWNING CAUSED BY POLYPHENOL OXIDASE [J].

EMBS, RJ ;

MARKAKIS, P .

JOURNAL OF FOOD SCIENCE, 1965, 30 (05) :753-&

[6]

Goetghebeur M, 1995, BIOTECHNOL APPL BIOC, V22, P315

[7] EFFECT OF ASCORBIC-ACID, SODIUM BISULFITE, AND THIOL COMPOUNDS ON MUSHROOM POLYPHENOL OXIDASE [J].

GOLANGOLDHIRSH, A ;

WHITAKER, JR .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1984, 32 (05) :1003-1009

[8] METALLOTHIONEIN [J].

HAMER, DH .

ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :913-951

[9]

HAREL E, 1967, ISRAEL J BOT, V16, P38

[10]

HUNZIKER PE, 1991, METHOD ENZYMOL, V205, P451

← 1 2 3 →