A new disposable microfluidic electrochemical paper-based device for the simultaneous determination of clinical biomarkers

被引：66
作者：
Cincotto, Fernando H. [1 ,2 ]
Fava, Elson L. [1 ]
Moraes, Fernando C. [1 ]
Fatibello-Filho, Orlando [1 ]
Faria, Ronaldo C. [1 ]
机构：
[1] Univ Fed Sao Carlos, Chem Dept, BR-13565905 Sao Carlos, SP, Brazil
[2] Univ Fed Rio de Janeiro, Inst Chem, Rio De Janeiro, RJ, Brazil
来源：
TALANTA | 2019年 / 195卷
基金：
巴西圣保罗研究基金会;
关键词：
Disposable electrochemical device; Graphene quantum dots (GQDs); Creatininase; Simultaneous detection; Clinical samples; URIC-ACID; SARCOSINE OXIDASE; CREATININE; SENSOR; BIOSENSOR; IMMOBILIZATION; FABRICATION; ELECTRODE; SYSTEMS; CELLS;
D O I：
10.1016/j.talanta.2018.11.022
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new disposable microfluidic electrochemical paper-based device (ePAD) consisting of two spot sensors in the same working electrode for the simultaneous determination of uric acid and creatinine was developed. The spot 1 surface was modified with graphene quantum dots for direct uric acid oxidation and spot 2 surface modified with graphene quantum dots, creatininase and a ruthenium electrochemical mediator for creatinine oxidation. The ePAD was employed to construct an electrochemical sensor (based on square wave voltammetry analysis) for the simultaneous determination of uric acid and creatinine in the 0.010-3.0 mu mol L-1 range. The device showed excellent analytical performance with a very low simultaneous detection limit of 8.4 nmol L-1 to uric acid and 3.7 nmol L-1 to creatinine and high selectivity. The ePAD was applied to the rapid and successful determination of those clinical biomarkers in human urine samples.
引用
收藏
页码：62 / 68
页数：7
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