A new disposable microfluidic electrochemical paper-based device for the simultaneous determination of clinical biomarkers

被引：65
作者：
Cincotto, Fernando H. [1 ,2 ]
Fava, Elson L. [1 ]
Moraes, Fernando C. [1 ]
Fatibello-Filho, Orlando [1 ]
Faria, Ronaldo C. [1 ]
机构：
[1] Univ Fed Sao Carlos, Chem Dept, BR-13565905 Sao Carlos, SP, Brazil
[2] Univ Fed Rio de Janeiro, Inst Chem, Rio De Janeiro, RJ, Brazil
来源：
TALANTA | 2019年 / 195卷
基金：
巴西圣保罗研究基金会;
关键词：
Disposable electrochemical device; Graphene quantum dots (GQDs); Creatininase; Simultaneous detection; Clinical samples; URIC-ACID; SARCOSINE OXIDASE; CREATININE; SENSOR; BIOSENSOR; IMMOBILIZATION; FABRICATION; ELECTRODE; SYSTEMS; CELLS;
D O I：
10.1016/j.talanta.2018.11.022
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new disposable microfluidic electrochemical paper-based device (ePAD) consisting of two spot sensors in the same working electrode for the simultaneous determination of uric acid and creatinine was developed. The spot 1 surface was modified with graphene quantum dots for direct uric acid oxidation and spot 2 surface modified with graphene quantum dots, creatininase and a ruthenium electrochemical mediator for creatinine oxidation. The ePAD was employed to construct an electrochemical sensor (based on square wave voltammetry analysis) for the simultaneous determination of uric acid and creatinine in the 0.010-3.0 mu mol L-1 range. The device showed excellent analytical performance with a very low simultaneous detection limit of 8.4 nmol L-1 to uric acid and 3.7 nmol L-1 to creatinine and high selectivity. The ePAD was applied to the rapid and successful determination of those clinical biomarkers in human urine samples.
引用
收藏
页码：62 / 68
页数：7
相关论文
共 35 条
  • [1] Simple and rapid fabrication of disposable carbon-based electrochemical cells using an electronic craft cutter for sensor and biosensor applications
    Afonso, Andre S.
    Uliana, Carolina V.
    Martucci, Diego H.
    Faria, Ronaldo C.
    [J]. TALANTA, 2016, 146 : 381 - 387
  • [2] Physics and applications of microfluidics in biology
    Beebe, DJ
    Mensing, GA
    Walker, GM
    [J]. ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2002, 4 : 261 - 286
  • [3] Self-contained microfluidic systems: a review
    Boyd-Moss, Mitchell
    Baratchi, Sara
    Di Venere, Martina
    Khoshmanesh, Khashayar
    [J]. LAB ON A CHIP, 2016, 16 (17) : 3177 - 3192
  • [4] Biomineralization Guided by Paper Templates
    Camci-Unal, Gulden
    Laromaine, Anna
    Hong, Estrella
    Derda, Ratmir
    Whitesides, George M.
    [J]. SCIENTIFIC REPORTS, 2016, 6
  • [5] High performance electrochemical sensors for dopamine and epinephrine using nanocrystalline carbon quantum dots obtained under controlled chronoamperometric conditions
    Canevari, Thiago C.
    Nakamura, Marcelo
    Cincotto, Fernando H.
    de Melo, Fernando M.
    Toma, Henrique E.
    [J]. ELECTROCHIMICA ACTA, 2016, 209 : 464 - 470
  • [6] Microfluidic chip integrated with flexible PDMS-based electrochemical cytosensor for dynamic analysis of drug-induced apoptosis on HeLa cells
    Cao, Jun-Tao
    Zhu, Ying-Di
    Rana, Rohit Kumar
    Zhu, Jun-Jie
    [J]. BIOSENSORS & BIOELECTRONICS, 2014, 51 : 97 - 102
  • [7] Microfluidic device for real-time formulation of reagents and their subsequent encapsulation into double emulsions
    Chang, Jui-Chia
    Swank, Zoe
    Keiser, Oliver
    Maerkl, Sebastian J.
    Amstad, Esther
    [J]. SCIENTIFIC REPORTS, 2018, 8
  • [8] A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis
    de Oliveira, Ricardo A. G.
    Camargo, Fiamma
    Pesquero, Naira C.
    Faria, Ronaldo Censi
    [J]. ANALYTICA CHIMICA ACTA, 2017, 957 : 40 - 46
  • [9] Versatility of divinylsulfone supports permits the tuning of CALB properties during its immobilization
    dos Santos, Jose C. S.
    Rueda, Nazzoly
    Sanchez, Alfredo
    Villalonga, Reynaldo
    Goncalves, Luciana R. B.
    Fernandez-Lafuente, Roberto
    [J]. RSC ADVANCES, 2015, 5 (45): : 35801 - 35810
  • [10] Rapid prototyping of microfluidic systems in poly(dimethylsiloxane)
    Duffy, DC
    McDonald, JC
    Schueller, OJA
    Whitesides, GM
    [J]. ANALYTICAL CHEMISTRY, 1998, 70 (23) : 4974 - 4984
1234