Mucin-chitosan complexes at the solid-liquid interface: Multilayer formation and stability in surfactant solutions

被引:80
作者
Dedinaite, A [1 ]
Lundin, M
Macakova, L
Auletta, T
机构
[1] Inst Surface Chem, SE-11486 Stockholm, Sweden
[2] Royal Inst Technol, Dept Chem, SE-10044 Stockholm, Sweden
关键词
D O I
10.1021/la0511844
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The adsorption of a biologically important glycoprotein, mucin, and mucin-chitosan complex layer formation on negatively charged surfaces, silica and mica, have been investigated employing ellipsometry, the interferometric surface apparatus, and atomic force microscopy techniques. Particular attention has been paid to the effect of an anionic surfactant sodium, dodecyl sulfate (SDS), with respect to the stability of the adsorption layers. It has been shown that mucin adsorbs on negatively charged surfaces to form highly hydrated layers. Such mucin layers readily associate with surfactants and are easily removed from the surfaces by rinsing with solutions of SDS at concentrations >= 0.2 cmc (1 cmc SDS in 30 mM NaCl is equal to 3.3 mM). The mucin adsorption layer is negatively charged, and we show how a positively charged polyelectrolyte, chitosan, associates with the preadsorbed mucin to form mucin-chitosan complexes that resist desorption by SDS even at SDS concentrations as high as 1 cmc. Thus, a method of mucin layer protection against removal by surfactants is offered. Further, we show how mucin-chitosan multilayers can be formed.
引用
收藏
页码:9502 / 9509
页数:8
相关论文
共 49 条
[1]   Salivary proteins: Protective and diagnostic value in cariology? [J].
Amerongen, AV ;
Bolscher, JGM ;
Veerman, ECI .
CARIES RESEARCH, 2004, 38 (03) :247-253
[2]   Interactions between mucin and alkyl sodium sulfates in solution. A light scattering study [J].
Bastardo, L ;
Claesson, P ;
Brown, W .
LANGMUIR, 2002, 18 (10) :3848-3853
[3]   Intraoral lubrication of PRP-1, statherin and mucin as studied by AFM [J].
Berg, CH ;
Lindh, L ;
Arnebrant, T .
BIOFOULING, 2004, 20 (01) :65-70
[4]   ATOMIC FORCE MICROSCOPE [J].
BINNIG, G ;
QUATE, CF ;
GERBER, C .
PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933
[5]  
CARLSTEDT I, 1985, ESSAYS BIOCHEM, V20, P40
[6]   Polyelectrolyte-surfactant association at solid surfaces [J].
Claesson, PM ;
Dedinaite, A ;
Blomberg, E ;
Sergeyev, VG .
BERICHTE DER BUNSEN-GESELLSCHAFT-PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 1996, 100 (06) :1008-1013
[7]   CHEMICAL ASPECTS OF MUCUS [J].
CLAMP, JR ;
ALLEN, A ;
GIBBONS, RA ;
ROBERTS, GP .
BRITISH MEDICAL BULLETIN, 1978, 34 (01) :25-41
[8]   Edible antimicrobial films based on chitosan matrix [J].
Coma, V ;
Martial-Gros, A ;
Garreau, S ;
Copinet, A ;
Salin, F ;
Deschamps, A .
JOURNAL OF FOOD SCIENCE, 2002, 67 (03) :1162-1169
[9]   Atomic force microscopy of gastric mucin and chitosan mucoadhesive systems [J].
Deacon, MP ;
McGurk, S ;
Roberts, CJ ;
Williams, PM ;
Tendler, SJB ;
Davies, MC ;
Davis, SS ;
Harding, SE .
BIOCHEMICAL JOURNAL, 2000, 348 :557-563
[10]   BUILDUP OF ULTRATHIN MULTILAYER FILMS BY A SELF-ASSEMBLY PROCESS .3. CONSECUTIVELY ALTERNATING ADSORPTION OF ANIONIC AND CATIONIC POLYELECTROLYTES ON CHARGED SURFACES [J].
DECHER, G ;
HONG, JD ;
SCHMITT, J .
THIN SOLID FILMS, 1992, 210 (1-2) :831-835