SV40 reporter viruses

被引：3
作者：
Katzman, Rebecca B.
Seeger, Mark
Rundell, Kathleen
机构：
[1] Northwestern Univ, Dept Microbiol Immunol, Chicago, IL 60611 USA
[2] Northwestern Univ, Robert H Lurie Comprehens Canc Ctr, Chicago, IL 60611 USA
来源：
JOURNAL OF VIROLOGICAL METHODS | 2008年 / 150卷 / 1-2期
关键词：
GFP-tagged SV40; FLAG-tagged SV40; superinfecting SV40;
D O I：
10.1016/j.jviromet.2008.02.013
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Three simian virus 40 (SV40) reporter viruses were constructed in this study. One expresses the green fluorescent protein (GFP) as a fusion protein with the first exon of large-T (IT) antigen and is useful for live-cell imaging. A second reporter virus has a FLAG epitope tag at the C-terminus of large-T antigen (vC-LTFLAG), and a third has the FLAG tag at the N-terminus of IT (vN-LTFLAG). The vC-LTFLAG construct grows to titers near those of wild-type (WT) virus and functions well as a reporter virus for SV40 infection. The vN-LTFLAG construct, while viable, has a defect in the production and spread of infectious particles. All three viruses are useful in detecting superinfecting virus in cells in which nuclear IT is already present, such as persistently infected human mesothelial cells. (C) 2008 Elsevier B.V. All rights reserved.
引用
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页码：7 / 13
页数：7
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