Localization of near-infrared contrast agents in tumors by intravital microscopy

被引：3

作者：

Becker, A ^{[1
]}

Schneider, G ^{[1
]}

Riefke, B ^{[1
]}

Licha, K ^{[1
]}

Semmler, W ^{[1
]}

机构：

[1] FU Berlin, Inst Diagnost Forsch, D-14050 Berlin, Germany

来源：

OPTICAL BIOPSIES AND MICROSCOPIC TECHNIQUES III, PROCEEDINGS OF | 1999年 / 3568卷

关键词：

intravital microscopy; fluorescence microscopy; cyanine dyes; optical contrast agents; near-infrared imaging;

D O I：

10.1117/12.336824

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this contribution we use intravital microscopy to study the dynamics of extravasation into normal and tumor tissue of several hydrophilic cyanine dyes used as near-infrared (NIR) contrast agents. The technique provides information about the angiographic properties of the dyes and about their interaction with tumor tissue under dynamic conditions in vivo. In our previous work we demonstrated that several NIR-absorbing fluorescent dyes enable in vivo fluorescence detection of tumors in mice and rats. However, the mechanism leading to dye accumulation and enhanced fluorescence in tumors is not fully understood. Increased extravasation of dyes into tumor tissue due to pathologically altered tumor vessels may be an important factor in this process. Indocyanine green (ICG) displayed predominantly intravascular distribution and rapid elimination resulting in enhanced fluorescence signal of vessels during the first 15 min after administration only. No elevated extravasation into tumor tissue was observed with ICG. A hydrophilic indotricarbocyanine derivative with a high molecular weight displayed prolonged intravascular distribution and increased fluorescence signal of the vasculature compared to surrounding tissue for up to five hours. Rapid extravasation and accumulation in tumor areas, yielding elevated contrast of tumors up to 15 min after administration, was observed with hydrophilic, low molecular weight indotricarbocyanine derivatives.

引用

页码：112 / 118

页数：7

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