Raman spectroscopy in investigations of mechanism of binding of human serum albumin to molecular probe fluorescein

被引：27

作者：

Vlasova, I. M. ^{[1
]}

Saletsky, A. M. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Phys, Moscow 119992, Russia

来源：

LASER PHYSICS LETTERS | 2008年 / 5卷 / 05期

关键词：

Raman spectroscopy; human serum albumin; protein binding Centers; fluorescein; protein conformation;

D O I：

10.1002/lapl.200810004

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The mechanism of binding of molecular probe fluorescein to to molecules of human serum albumin was studied by the Raman spectroscopy method. The position of binding Center on human serum albumin molecule for fluorescein is determined. The amino acid residues of albumin molecule, participating in binding of fluorescein at different pH values of solution, are established. The conformation rearrangements of globules of human serum albumin, taking place at binding of fluorescein at different pH values of solution, are registered.

引用

页码：384 / 389

页数：6

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