Use of 2 pneumococcal common protein real-time polymerase chain reaction assays in healthy children colonized with Streptococcus pneumoniae

被引：12

作者：

Rouphael, Nadine ^{[1
,2
]}

Steyn, Sanet ^{[3
]}

Bangert, Mathieu ^{[1
,4
]}

Sampson, Jackie S. ^{[1
]}

Adrian, Peter ^{[5
,6
]}

Madhi, Shabir A. ^{[5
,6
]}

Klugman, Keith P. ^{[5
,7
,8
]}

Ades, Edwin W. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Atlanta, GA 30329 USA

[2] Emory Univ, Sch Med, Atlanta, GA 30303 USA

[3] Emory Univ, Atlanta, GA 30322 USA

[4] Univ Liverpool, Liverpool Sch Trop Med, Liverpool L3 5QA, Merseyside, England

[5] Univ Witwatersrand, MRC, Resp & Meningeal Pathogens Res Unit, ZA-2131 Johannesburg, South Africa

[6] Univ Witwatersrand, Natl Res Fdn, Dept Sci & Technol, ZA-2013 Johannesburg, South Africa

[7] Emory Univ, Rollins Sch Publ Hlth, Hubert Dept Global Hlth, Atlanta, GA 30329 USA

[8] Emory Univ, Sch Med, Div Infect Dis, Atlanta, GA 30329 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2011年 / 70卷 / 04期

关键词：

lytA and psaA; PCR assay; Streptococcus pneumoniae; CONJUGATE VACCINE; IDENTIFICATION;

D O I：

10.1016/j.diagmicrobio.2010.09.006

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Polymerase chain reaction (PCR) offers promise in pneumococcal diagnostics, but whether nasopharyngeal carriage causes false-positive results in people colonized with Streptococcus pneumoniae is unknown. We found in serum no positive samples for pneumococcal DNA in 100 carriers and noncarriers using 2 different real-time PCR assays targeting lytA and psaA genes. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：452 / 454

页数：3

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