Use of 2 pneumococcal common protein real-time polymerase chain reaction assays in healthy children colonized with Streptococcus pneumoniae

被引：12
|
作者：
Rouphael, Nadine [1 ,2 ]
Steyn, Sanet [3 ]
Bangert, Mathieu [1 ,4 ]
Sampson, Jackie S. [1 ]
Adrian, Peter [5 ,6 ]
Madhi, Shabir A. [5 ,6 ]
Klugman, Keith P. [5 ,7 ,8 ]
Ades, Edwin W. [1 ]
机构：
[1] Ctr Dis Control & Prevent, Atlanta, GA 30329 USA
[2] Emory Univ, Sch Med, Atlanta, GA 30303 USA
[3] Emory Univ, Atlanta, GA 30322 USA
[4] Univ Liverpool, Liverpool Sch Trop Med, Liverpool L3 5QA, Merseyside, England
[5] Univ Witwatersrand, MRC, Resp & Meningeal Pathogens Res Unit, ZA-2131 Johannesburg, South Africa
[6] Univ Witwatersrand, Natl Res Fdn, Dept Sci & Technol, ZA-2013 Johannesburg, South Africa
[7] Emory Univ, Rollins Sch Publ Hlth, Hubert Dept Global Hlth, Atlanta, GA 30329 USA
[8] Emory Univ, Sch Med, Div Infect Dis, Atlanta, GA 30329 USA
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2011年 / 70卷 / 04期
关键词：
lytA and psaA; PCR assay; Streptococcus pneumoniae; CONJUGATE VACCINE; IDENTIFICATION;
D O I：
10.1016/j.diagmicrobio.2010.09.006
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Polymerase chain reaction (PCR) offers promise in pneumococcal diagnostics, but whether nasopharyngeal carriage causes false-positive results in people colonized with Streptococcus pneumoniae is unknown. We found in serum no positive samples for pneumococcal DNA in 100 carriers and noncarriers using 2 different real-time PCR assays targeting lytA and psaA genes. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：452 / 454
页数：3
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