Revisiting the taxonomy of the genus Elizabethkingia using whole-genome sequencing, optical mapping, and MALDI-TOF, along with proposal of three novel Elizabethkingia species: Elizabethkingia bruuniana sp nov., Elizabethkingia ursingii sp nov., and Elizabethkingia occulta sp nov.

被引：77

作者：

Nicholson, Ainsley C. ^{[1
]}

Gulvik, Christopher A. ^{[1
]}

Whitney, Anne M. ^{[1
]}

Humrighouse, Ben W. ^{[1
]}

Graziano, James ^{[1
]}

Emery, Brian ^{[1
]}

Bell, Melissa ^{[1
]}

Loparev, Vladimir ^{[2
]}

Juieng, Phalasy ^{[2
]}

Gartin, Jarrett ^{[1
]}

Bizet, Chantal ^{[3
]}

Clermont, Dominique ^{[3
]}

Criscuolo, Alexis ^{[4
]}

Brisse, Sylvain ^{[5
,6
,7
]}

McQuiston, John R. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div High Consequence Pathogens & Pathol, Bacterial Special Pathogens Branch, Special Bacteriol Reference Lab, Atlanta, GA 30333 USA

[2] Ctr Dis Control & Prevent, Div Sci Resources, Atlanta, GA 30333 USA

[3] Inst Pasteur, CIP, Dept Microbiol, Paris, France

[4] Inst Pasteur, USR IP CNRS 3756, Bioinformat & Biostat Hub C3BI, Paris, France

[5] Inst Pasteur, Microbial Evolutionary Genom, Paris, France

[6] CNRS, UMR 3525, Paris, France

[7] Inst Pasteur, Biodivers & Epidemiol Bacterial Pathogens, Paris, France

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2018年 / 111卷 / 01期

关键词：

AAI; ANI; Elizabethkingia; MALDI-TOF; SNPs; Taxonomy; DNA-DNA HYBRIDIZATION; CHRYSEOBACTERIUM-MENINGOSEPTICUM; SPHINGOBACTERIUM-MIZUTAE; SUBJECTIVE SYNONYM; FLAVOBACTERIUM; ALGORITHM; PATHOGEN; BACTEREMIA; MIRICOLA; STRAINS;

D O I：

10.1007/s10482-017-0926-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genus Elizabethkingia is genetically heterogeneous, and the phenotypic similarities between recognized species pose challenges in correct identification of clinically derived isolates. In addition to the type species Elizabethkingia meningoseptica, and more recently proposed Elizabethkingia miricola, Elizabethkingia anophelis and Elizabethkingia endophytica, four genomospecies have long been recognized. By comparing historic DNA-DNA hybridization results with whole genome sequences, optical maps, and MALDI-TOF mass spectra on a large and diverse set of strains, we propose a comprehensive taxonomic revision of this genus. Genomospecies 1 and 2 contain the type strains E. anophelis and E. miricola, respectively. Genomospecies 3 and 4 are herein proposed as novel species named as Elizabethkingia bruuniana sp. nov. (type strain, G0146(T) = DSM 2975(T) = CCUG 69503(T) = CIP 111191(T)) and Elizabethkingia ursingii sp. nov. (type strain, G4122(T) = DSM 2974(T) = CCUG 69496(T) = CIP 111192(T)), respectively. Finally, the new species Elizabethkingia occulta sp. nov. (type strain G4070(T) = DSM 2976(T) = CCUG 69505(T) = CIP 111193(T)), is proposed.

引用

页码：55 / 72

页数：18

共 61 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].