Detection and identification of Candida spp. in human serum by LightCycler® real-time polymerase chain reaction

被引：37

作者：

Dunyach, Catherine ^{[1
]}

Bertout, Sebastien ^{[1
]}

Phelipeau, Cecile ^{[2
]}

Drakulovski, Pascal ^{[1
]}

Reynes, Jacques ^{[3
]}

Mallie, Michele ^{[1
]}

机构：

[1] Fac Pharm Montpellier, VIH SIDA Maladies Assoc, UMR 145 IRD UMI, Lab Parasitol & Mycol Med, F-34093 Montpellier, France

[2] CHU Bordeaux, Parasitol Lab, Hosp St Andre, F-33075 Bordeaux, France

[3] CHU Guy De Chauliac, Serv Maladies Infect & Trop, F-34295 Montpellier 5, France

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 60卷 / 03期

关键词：

diagnosis; systemic candidiasis; real-time PCR;

D O I：

10.1016/j.diagmicrobio.2007.09.014

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The aim of this work was to develop LightCycler (R) real-time polymerase chain reaction method to allow rapid detection and identification of Candida spp. in human serum with panfungal primers (internal transcribed spacer [ITS] and L18). Melting-curve analysis of the ITS sequences showed that each amplicon presented a specific melting point and enabled identification of 5 Candida spp. After parameters optimization, 58 sera were preliminary analyzed from 23 patients. For L18 primers, the LightCycler (R) system enabled detection of DNA in 92% of patients with positive blood culture. These primers were not able to differentiate between species of Candida. By using ITS primers, the LightCycler (R) system enabled detection of DNA in sera from 76.9% of patients with positive blood culture. With ITS primers, the species responsible for the infection was identified for 11 patients. These data revealed the LightCycler (R) as a potential tool for early detection and identification of Candida. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：263 / 271

页数：9

共 30 条

[1] The epidemiology of hematogenous candidiasis caused by different Candida species [J].

AbiSaid, D ;

Anaissie, E ;

Uzun, O ;

Raad, I ;

Pinzcowski, H ;

Vartivarian, S .

CLINICAL INFECTIOUS DISEASES, 1997, 24 (06) :1122-1128

[2] Seminested PCR for diagnosis of candidemia: Comparison with culture, antigen detection, and biochemical methods for species identification [J].

Ahmad, S ;

Khan, Z ;

Mustafa, AS ;

Khan, ZU .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (07) :2483-2489

[3] Epidemiology and predictors of mortality in cases of Candida bloodstream infection:: Results from population-based surveillance, Barcelona, Spain, from 2002 to 2003 [J].

Almirante, B ;

Rodríguez, D ;

Park, BJ ;

Cuenca-Estrella, M ;

Planes, AM ;

Almela, M ;

Mensa, J ;

Sanchez, F ;

Ayats, J ;

Gimenez, M ;

Saballs, P ;

Fridkin, SK ;

Morgan, J ;

Rodriguez-Tudela, JL ;

Warnock, DW ;

Pahissa, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (04) :1829-1835

[4] Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: An international consensus [J].

Ascioglu, S ;

Rex, JH ;

de Pauw, B ;

Bennett, JE ;

Bille, J ;

Crokaert, F ;

Denning, DW ;

Donnelly, JP ;

Edwards, JE ;

Erjavec, Z ;

Fiere, D ;

Lortholary, O ;

Maertens, J ;

Meis, JF ;

Patterson, TF ;

Ritter, J ;

Selleslag, D ;

Shah, PM ;

Stevens, DA ;

Walsh, TJ .

CLINICAL INFECTIOUS DISEASES, 2002, 34 (01) :7-14

[5] Serum is more suitable than whole blood for diagnosis of systemic candidiasis by nested PCR [J].

Bougnoux, ME ;

Dupont, C ;

Mateo, J ;

Saulnier, P ;

Faivre, V ;

Payen, D ;

Nicolas-Chanoine, MH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (04) :925-930

[6] Towards a molecular diagnosis of invasive aspergillosis and disseminated candidosis [J].

Bretagne, S ;

Costa, JM .

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2005, 45 (03) :361-368

[7] Monochrome LightCycler PCR assay for detection and quantification of five common species of Candida and Aspergillus [J].

Bu, R ;

Sathiapalan, RK ;

Ibrahim, MM ;

Al-Mohsen, I ;

Almodavar, E ;

Gutierrez, MI ;

Bhatia, K .

JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (03) :243-248

[8] Rapid identification of yeasts in positive blood cultures by a multiplex PCR method [J].

Chang, HC ;

Leaw, SN ;

Huang, AH ;

Wuk, TL ;

Chang, TC .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (10) :3466-3471

[9] Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR [J].

Corless, CE ;

Guiver, M ;

Borrow, R ;

Edwards-Jones, V ;

Fox, AJ ;

Kaczmarski, EB .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (04) :1553-1558

[10] Real-time PCR coupled with automated DNA extraction and detection of galactomannan antigen in serum by enzyme-linked immunosorbent assay for diagnosis of invasive aspergillosis [J].

Costa, C ;

Costa, JM ;

Desterke, C ;

Botterel, F ;

Cordonnier, C ;

Bretagne, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (06) :2224-2227

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