Improvement of the Lipase Immobilization Procedure on Monodispersed Fe3O4 Magnetic Nanoparticles

Here we report on the immobilization of lipase (E.C 3.1.1.1) from Thermomyces lanuginosa on monodispersed Fe3O4 nanoparticles. The nanoparticles were prepared by a bottom-up chemical strategy based on low temperature thermal decomposition of an iron precursor in organic solvent in the presence of surfactants. It offers many advantages, such as experimental easiness, potential low-cost fabrication, very high nanoparticles size control and reproducibility. The nanoparticles were treated with (3-aminopropyl) triethoxysilane (APTES) and then activated via the glutaraldehyde method to couple with lipase by a covalent bond. High recovered activity (77%) was obtained, a new washing procedure was developed to double the immobilization efficiency.