Recent progress in microchip electrophoresis-mass spectrometry

被引：31

作者：

Kitagawa, Fumihiko ^{[1
]}

Otsuka, Koji ^{[1
]}

机构：

[1] Kyoto Univ, Dept Chem Mat, Grad Sch Engn, Nishikyo Ku, Kyoto 6158510, Japan

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2011年 / 55卷 / 04期

关键词：

Microchip electrophoresis; Mass spectrometry; Electrospray ionization; Laser desorption ionization; Peptides; LEVEL PROTEIN DIGESTS; CAPILLARY-ELECTROPHORESIS; MICROFABRICATED DEVICES; MICROFLUIDIC DEVICES; ELECTROKINETIC CHROMATOGRAPHY; ELECTROSPRAY EMITTER; INORGANIC ANIONS; HIGH-THROUGHPUT; HIGH-EFFICIENCY; ORGANIC-ACIDS;

D O I：

10.1016/j.jpba.2010.11.013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This review highlights the methodological and instrumental developments in microchip electrophoresis (MCE)-mass spectrometry (MS) from 1997. In MCE-MS, the development of ionization interface is one of the most important issues to realize highly sensitive detection and high separation efficiency. Among several interfaces, electrospray ionization (ESI) has been mainly employed to MCE-MS since a simple structure of the ESI interface is suitable for coupling with the microchips. Although the number of publications is still limited, laser desorption ionization (LDI) interface has also been developed for MCE-MS. The characteristics of the ESI and LDI interfaces applied to the electrophoresis microchips are presented in this review. The scope of applications in MCE-MS covers mainly biogenic compounds such as bioactive amines, peptides, tryptic digests and proteins. This review provides a comprehensive table listing the applications in MCE-MS. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：668 / 678

页数：11

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