Separation of eight bedaquiline analogue diastereomers by HPLC on an immobilized polysaccharide-based chiral stationary phase

被引：9

作者：

Jiang, Shenmeng ^{[1
]}

Wu, Chengjun ^{[1
]}

Yu, Jia ^{[1
]}

Sun, Tiemin ^{[2
]}

Guo, Xingjie ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Sch Pharm, 103 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China

[2] Shenyang Pharmaceut Univ, Sch Pharmaceut Engn, Shenyang, Liaoning, Peoples R China

来源：

CHIRALITY | 2019年 / 31卷 / 01期

关键词：

bedaquiline analogues; Chiralpak IC; diastereoseparation; separation mechanism; thermodynamic study; CHROMATOGRAPHY; RESOLUTION;

D O I：

10.1002/chir.23037

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The high-performance liquid chromatography (HPLC) is a powerful method in the area of stereoisomer separation. In this study, separation of eight bedaquiline analogue diastereomers (compounds 1-8) was first examined on a cellulose tris-(3,5-dichlorophenylcarbamate) chiral stationary phase, ie, Chiralpak IC in the normal phase mode. The influences of organic modifier types, alcohol content, and column temperature on diastereoseparation were evaluated. Under the optimum chromatographic conditions, all the analyte stereoisomers were successfully separated. The experimental results revealed the great influence of analytes' structures on the diastereoseparation with Chiralpak IC. In addition, thermodynamic parameters were calculated by Van't Hoff plots, and correlative chiral recognition mechanisms were discussed further.

引用

页码：72 / 78

页数：7

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