O-Glycosylated Cell Wall Proteins Are Essential in Root Hair Growth

被引：243

作者：

Velasquez, Silvia M. ^{[1
]}

Ricardi, Martiniano M. ^{[1
]}

Dorosz, Javier Gloazzo ^{[1
]}

Fernandez, Paula V. ^{[2
]}

Nadra, Alejandro D. ^{[3
]}

Pol-Fachin, Laercio ^{[4
]}

Egelund, Jack ^{[5
]}

Gille, Sascha ^{[6
]}

Harholt, Jesper ^{[5
]}

Ciancia, Marina ^{[2
]}

Verli, Hugo ^{[4
]}

Pauly, Markus ^{[6
]}

Bacic, Antony ^{[7
]}

Olsen, Carl Erik ^{[8
]}

Ulvskov, Peter ^{[5
]}

Petersen, Bent Larsen ^{[5
]}

Somerville, Chris ^{[6
]}

Iusem, Norberto D. ^{[1
,9
]}

Estevez, Jose M. ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Consejo Nacl Invest Cient & Tecn IFIByNE CONICET, Inst Fisiol Biol Mol & Neurociencias, Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Fac Agron, Ctr Invest Hidratos Carbono CONICET, Dept Biol Aplicada & Alimentos,Catedra Quim Biomo, Buenos Aires, DF, Argentina

[3] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Biol, Buenos Aires, DF, Argentina

[4] Univ Fed Rio Grande do Sul, Fac Farm, BR-90610000 Porto Alegre, RS, Brazil

[5] Univ Copenhagen, Fac Life Sci, Dept Plant Biol & Biotechnol, DK-1871 Copenhagen, Denmark

[6] Univ Calif Berkeley, Energy Biosci Inst, Berkeley, CA 94720 USA

[7] Univ Melbourne, Sch Bot, Plant Cell Biol Res Ctr, Melbourne, Vic 3010, Australia

[8] Univ Copenhagen, Fac Life Sci, Dept Nat Sci, DK-1871 Copenhagen, Denmark

[9] Univ Buenos Aires, Fac Ciencias Exactas & Nat, LFBM, Dept Fisiol Biol Mol & Celular, Buenos Aires, DF, Argentina

来源：

SCIENCE | 2011年 / 332卷 / 6036期

基金：

澳大利亚研究理事会;

关键词：

MOLECULAR CHARACTERIZATION; PROLYL; 4-HYDROXYLASE; ARABIDOPSIS; EXTENSIN; GENES; CODES; IDENTIFICATION; REQUIRES; MUTANTS; TOBACCO;

D O I：

10.1126/science.1206657

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.

引用

页码：1401 / 1403

页数：3

共 31 条

[1] DIPYRIDYL-INDUCED CELL ELONGATION AND INHIBITION OF CELL WALL HYDROXYPROLINE BIOSYNTHESIS [J].