Poly-2-methyl-2-oxazoline:: A peptide-like polymer for protein-repellent surfaces

被引:298
作者
Konradi, Rupert [1 ]
Pidhatika, Bidhari [1 ]
Muehlebach, Andreas [2 ]
Textort, Marcus [1 ]
机构
[1] ETH, Dept Mat, Surface Sci & Technol Lab, CH-8093 Zurich, Switzerland
[2] Ciba, Res Grp, CH-4002 Basel, Switzerland
关键词
D O I
10.1021/la702917z
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Surface coatings with so-called protei n-repellent or nonfouling polymers have become indispensable for the development of modern therapeutic and diagnostic medical devices such as biosensors, drug-delivery capsules, and biomedical implants. Nowadays, poly(ethylene glycol) (PEG) is routinely used for these purposes. However, there is increasing evidence that PEG has limited long-term stability, particularly in vivo. Here we investigate poly(2-methyl-2-oxazoline) (PMOXA) as a potential alternative polymer. We designed comb copolymers consisting of a polycationic poly(L-lysine) backbone and PMOXA side chains by analogy to precisely studied and highly protein-repellent PEG-based systems. Using optical waveguide lightmode spectroscopy, we quantitatively compare the in situ self-assembly of the comb copolymers on negatively charged surfaces and the exposure of the formed monolayers to full human serum. We find that the PMOXA-based coatings with an optimal side-chain grafting density eliminate protein adsorption to a level of <2 ng/cm(2); that is, they quantitatively equal the protein-repellent properties of the best PEG-based coatings.
引用
收藏
页码:613 / 616
页数:4
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