Purification of antibody against Ara h 2 by a homemade immunoaffinity chromatography column

被引：3

作者：

Wu, Zhihua ^{[1
,2
]}

Li, Kun ^{[1
,3
]}

Zhan, Shaode ^{[1
,4
]}

Tong, Ping ^{[1
,4
]}

Li, Xin ^{[1
,4
]}

Yang, Anshu ^{[1
,2
]}

Chen, Hongbing ^{[1
,2
]}

机构：

[1] Nanchang Univ, State Key Lab Food Sci & Technol, 235 Nanjing Rd East, Nanchang 330047, Jiangxi, Peoples R China

[2] Nanchang Univ, Sino German Joint Res Inst, Nanchang, Jiangxi, Peoples R China

[3] Nanchang Univ, Environm & Chem Sch, Nanchang, Jiangxi, Peoples R China

[4] Nanchang Univ, Sch Food Sci & Technol, Nanchang, Jiangxi, Peoples R China

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2017年 / 47卷 / 08期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

Ara h 2; IAC; immunoaffinity; MPG; PcAb; purification; ACTIVATED SEPHAROSE; IMMUNOGLOBULIN-G; VOID-EXCLUSION; PROTEINS; PEANUT; EXTRACTION; TOOL; ALLERGENICITY; THERAPY; BINDING;

D O I：

10.1080/10826068.2017.1342265

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies are used extensively in numerous applications both in vivo and in vitro. To purify anti-Ara h 2 polyclonal antibody, a homemade immunoaffinity chromatography (IAC) column method was established. The properties of homemade column were compared with those of the mAb affinity protein G (MPG) agarose high flow, a commercially available column successfully used in capturing polyclonal antibodies. During antibody purification from rabbits' antiserum against Ara h 2, the column capacity, recovery, and purification factor were characterized for IAC and MPG. The homemade IAC could separate the corresponding antibody with higher specificity and lower cost but with lower recovery and column capacity than those of MPG. Thus, the homemade IAC is a specific, inexpensive, and suitable method that can be used for various laboratory purifications.

引用

页码：841 / 846

页数：6

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