Selective media for the specific isolation and enumeration of Botrytis cinerea conidia

被引：53

作者：

Edwards, SG ^{[1
]}

Seddon, B ^{[1
]}

机构：

[1] Univ Aberdeen, Dept Agr, Aberdeen AB24 5UA, Scotland

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2001年 / 32卷 / 02期

关键词：

D O I：

10.1046/j.1472-765x.2001.00857.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: To develop selective media for the enumeration of Botrytis cinerea. Methods and Results: Two new media, Botrytis Selective Medium (BSM) and Botrytis Spore Trap Medium (BSTM), were developed and compared with currently available media for the enumeration of B. cinerea conidia from the environment. The new Botrytis media proved advantageous over previous media because they were easier to prepare, had greater selectivity and allowed enumeration when a greater number of colony-forming units were present on individual plates. Conclusions: BSM and BSTM were shown to be suitable selective media for the enumeration of B. cinerea conidia from the environment. Significance and Impact of the Study: The media developed can be used to monitor the population of the pathogen B. cinerea and will allow detailed studies within the crop environments.

引用

页码：63 / 66

页数：4

共 12 条

[1]

Coley-Smith J.R., 1980, The Biology of Botrytis

[2]

EDWARDS SG, 1993, THESIS U ABERDEEN

[3] Biological control of Botrytis-incited diseases and powdery mildews in greenhouse crops [J].