Determination of anti-idiotype antibodies by surface plasmon resonance

被引：9
作者：
Haimovich, J
Czerwinski, D
Wong, CP
Levy, R
机构：
[1] Tel Aviv Univ, Sackler Sch Med, Dept Human Microbiol, IL-09978 Tel Aviv, Israel
[2] Stanford Univ, Med Ctr, Sch Med, Div Med Oncol, Stanford, CA 94305 USA
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1998年 / 214卷 / 1-2期
关键词：
tumour immunotherapy; B cells; monoclonal and polyclonal antibodies;
D O I：
10.1016/S0022-1759(98)00051-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method based on surface plasmon resonance has been used to detect and to quantitate antibodies against other antibodies. This method has been applied to the detection of syngeneic antibodies. Concentrations as low as 0.3 mu g/ml could be detected even when the antibodies were present in undiluted serum. Quantitation of both monoclonal and polyclonal antibodies could be performed. Anti-idiotype antibodies could be detected equally well against an IgM molecule derived directly from the B cell tumour or against a chimeric protein composed of the same idiotypic determinants embedded in a human IgG molecule. Surface plasmon resonance will be the method of choice for the detection of antibody responses in humans vaccinated against idiotypic determinants of their own tumour. (C) 1998 Elsevier Science B.V. All rights reserved.
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收藏
页码：113 / 119
页数：7
相关论文
共 13 条
[1]  
BERGMAN Y, 1977, EUR J IMMUNOL, V7, P317
[2]  
CAMPBELL MJ, 1987, J IMMUNOL, V139, P2825
[3]  
CHEN TT, 1994, J IMMUNOL, V153, P4775
[4]  
ESHHAR Z, 1979, J IMMUNOL, V122, P2430
[5]   Tumor-specific idiotype vaccines in the treatment of patients with B-cell lymphoma - Long-term results of a clinical trial [J].
Hsu, FJ ;
Caspar, CB ;
Czerwinski, D ;
Kwak, LW ;
Liles, TM ;
Syrengelas, A ;
TaidiLaskowski, B ;
Levy, R .
BLOOD, 1997, 89 (09) :3129-3135
[6]   IMMOBILIZATION OF PROTEINS TO A CARBOXYMETHYLDEXTRAN-MODIFIED GOLD SURFACE FOR BIOSPECIFIC INTERACTION ANALYSIS IN SURFACE-PLASMON RESONANCE SENSORS [J].
JOHNSSON, B ;
LOFAS, S ;
LINDQUIST, G .
ANALYTICAL BIOCHEMISTRY, 1991, 198 (02) :268-277
[7]  
KAMINSKI MS, 1987, J IMMUNOL, V138, P1289
[8]   ANALYSIS OF ACTIVE ANTIBODY CONCENTRATION - SEPARATION OF AFFINITY AND CONCENTRATION PARAMETERS [J].
KARLSSON, R ;
FAGERSTAM, L ;
NILSHANS, H ;
PERSSON, B .
JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 166 (01) :75-84
[9]   INDUCTION OF IMMUNE-RESPONSES IN PATIENTS WITH B-CELL LYMPHOMA AGAINST THE SURFACE-IMMUNOGLOBULIN IDIOTYPE EXPRESSED BY THEIR TUMORS [J].
KWAK, LW ;
CAMPBELL, MJ ;
CZERWINSKI, DK ;
HART, S ;
MILLER, RA ;
LEVY, R .
NEW ENGLAND JOURNAL OF MEDICINE, 1992, 327 (17) :1209-1215
[10]   MONOCLONAL ANTI-IDIOTYPE ANTIBODIES AGAINST THE MURINE B-CELL LYMPHOMA 38C13 - CHARACTERIZATION AND USE AS PROBES FOR THE BIOLOGY OF THE TUMOR INVIVO AND INVITRO [J].
MALONEY, DG ;
KAMINSKI, MS ;
BUROWSKI, D ;
HAIMOVICH, J ;
LEVY, R .
HYBRIDOMA, 1985, 4 (03) :191-209
12