P38 is involved in immune response by regulating inflammatory cytokine expressions in the Pacific oyster Crassostrea gigas

被引：29
作者：
Sun, Jiejie [1 ,3 ]
Wang, Lingling [1 ,2 ,3 ]
Wu, Zhaojun [1 ,3 ]
Han, Shuo [1 ,3 ]
Wang, Liyan [1 ,3 ]
Li, Meijia [1 ,3 ]
Liu, Zhaoqun [1 ,3 ]
Song, Linsheng [1 ,2 ,3 ]
机构：
[1] Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China
[3] Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
来源：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2019年 / 91卷
基金：
美国国家科学基金会;
关键词：
Oysters; Mitogen-activated protein kinase; Interleukins; Lipopolysaccharide; ACTIVATED PROTEIN-KINASE; NF-KAPPA-B; TNF-ALPHA; MOLECULAR CHARACTERIZATION; GENE-EXPRESSION; MAP KINASE; PATHWAY; INFECTION; CGIL17-5; CLONING;
D O I：
10.1016/j.dci.2018.10.011
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
P38 mitogen-activated protein kinases are serine/threonine protein kinases reportedly involved in the innate immunity of vertebrates and invertebrates. In the present study, a P38 homolog (CgP38) was characterized from the Pacific oyster Crassostrea gigas. The full-length cDNA of CgP38 was of 1515 bp containing a 1101 bp open reading frame. A serine/threonine protein kinase (S_TKc) domain with a conserved Thr-Gly-Tyr motif and an ATRW substrate-binding site was found in the deduced amino acid sequence of CgP38. CgP38 shared a close evolutionary relationship with ChP38 from the Hong Kong oyster Crassostrea hongkongensis. The transcript levels of CgP38 in hemocytes increased significantly from 12 h to 48 h after lipopolysaccharide (LPS) stimulation and from 12 h to 24 h after Vibrio splendidus stimulation. The phosphorylation level of CgP38 in oyster hemocytes increased significantly at 2 h after LPS stimulation. CgP38 positively regulated the expression of interleukins, such as CgIL17-1, CgIL17-2, CgIL17-3, CgIL17-4 and CgIL17-6, and tumor necrosis factor CgTNF after LPS or V. splendidns stimulation. These results suggested that CgP38 participated in oyster immune response by regulating the expressions of inflammatory cytokines.
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收藏
页码：108 / 114
页数：7
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