Direct detection of Exophiala and Scedosporium species in sputa of patients with cystic fibrosis

被引：17

作者：

Chen, Min ^{[1
,2
,3
]}

Kondori, Nahid ^{[4
]}

Deng, Shuwen ^{[8
]}

van den Ende, A. H. G. Gerrits ^{[2
]}

Lackner, M. ^{[5
]}

Liao, Wanqing ^{[1
]}

de Hoog, G. S. ^{[1
,2
,3
,6
,7
]}

机构：

[1] Second Mil Med Univ, Shanghai Changzheng Hosp, Shanghai Key Lab Mol Med Mycol, Dept Dermatol,Shanghai Inst Med Mycol, Shanghai 200003, Peoples R China

[2] Westerdijk Fungal Biodivers Inst, Utrecht, Netherlands

[3] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands

[4] Univ Gothenburg, Sahlgrenska Acad, Dept Infect Dis, Gothenburg, Sweden

[5] Med Univ Innsbruck, Div Hyg & Med Microbiol, Innsbruck, Austria

[6] Peking Univ, Hosp 1, Res Ctr Med Mycol, Beijing, Peoples R China

[7] Univ Parana, Dept Basic Biol, Curitiba, Parana, Brazil

[8] Peoples Hosp Suzhou, Natl New & Hitech Ind Dev Zone, Dept Med Microbiol, Suzhou, Jiangsu, Peoples R China

来源：

MEDICAL MYCOLOGY | 2018年 / 56卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Exophiala dermatitidis; Scedosporium; cystic fibrosis; sputum; MOLECULAR EPIDEMIOLOGY; FILAMENTOUS FUNGI; CULTURE-MEDIUM; DERMATITIDIS; APIOSPERMUM; PREVALENCE; PSEUDALLESCHERIA; AMPLIFICATION; INFECTION; COMPLEX;

D O I：

10.1093/mmy/myx108

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Detection of species of Exophiala and Scedosporium in the respiratory tracts of cystic fibrosis (CF) patients remains controversial because of highly variable results. The results of our study suggested a significantly higher prevalence and more complex colonization than previously estimated. Approximately 17% (27/162) of clinical sputum samples were found to be positive for Exophiala dermatitidis and 30% (49/162) were positive for Scedosporium apiospermum / S. boydii species complex determined by reverse line blot (RLB) hybridization. In contrast, only 14.2% (23/162) and 1.2% ( 2 / 1 62) of clinical sputa were positive for E. dermatitidis and S. apiospermum / S. boydii species complex when tested by culture, respectively. Molecular detection methods, such as loop-mediated isothermal amplification (LAMP) or reverse line blot (RLB) hybridization, have the potential to become powerful alternatives to selective culture, providing a more realistic understanding on the prevalence of E. dermatitidis and S. apiospermum / S. boydii species complex in the respiratory tract of CF patients.

引用

页码：695 / 702

页数：8