Clinical responses in cows vaccinated with a developed prototype killed Staphylococcus aureus mastitis vaccine

被引:2
作者
Hambali, I. U. [1 ,3 ]
Bhutto, K. R. [1 ]
Jesse, F. F. A. [1 ,2 ,7 ]
Lawan, A. [1 ,4 ]
Odhah, M. N. [1 ,5 ]
Wahid, A. H. [1 ]
Azmi, M. L. Mohd [6 ]
Zakaria, Z. [6 ]
Arsalan, M. [7 ,8 ]
Muhammad, N. A. [7 ,9 ]
Jefri, M. N. [1 ]
机构
[1] Univ Putra Malaysia, Dept Vet Clin Studies, Fac Vet Med, Serdang 43400, Selangor, Malaysia
[2] Univ Putra Malaysia, Res Ctr Ruminant Dis, Serdang 43400, Selangor, Malaysia
[3] Univ Maiduguri, Dept Vet Publ Hlth & Prevent Med, Maiduguri 600233, Nigeria
[4] Univ Maiduguri, Dept Vet Med, Fac Vet Med, Maiduguri, Nigeria
[5] Thamar Univ, Fac Agr & Vet Med, Dept Vet Med, Dhamar 39, Yemen
[6] Univ Putra Malaysia, Dept Vet Pathol & Microbiol, Fac Vet Med, Serdang 43400, Selangor, Malaysia
[7] Univ Putra Malaysia, Inst Trop Agr & Food Secur, Serdang 43400, Selangor, Malaysia
[8] Livestock & Dairy Dev Dept Baluchistan, Directorate Anim Hlth, Balochistan 87300, Pakistan
[9] Univ Vet & Anim Sci Lahore, Dept Clin Med & Surg, Lahore 54500, Pakistan
关键词
Mastitis; S; aureus; Vaccine; Clinical response; Cow; Malaysia; SUBCLINICAL MASTITIS; BIOCHEMISTRY RESPONSES; RAW-MILK; B; DAIRY; PREVALENCE; LIPOPOLYSACCHARIDE; ANTIBIOTICS; ASSOCIATION; TEMPERATURE;
D O I
10.1016/j.micpath.2018.08.017
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Mastitis is an inflammatory condition of the udder that occurs as a result of the release of leucocytes into the udder in a response to bacterial invasion. The major causes of mastitis are an array of gram positive and negative bacteria, however, algae, virus, fungi, mechanical or thermal injury to the gland have also been identified as possible causes. Mastitis vaccines are yet to be developed using Malaysian local isolate of bacteria. The objective of the present experimental trial was to develop a monovalent vaccine against mastitis using S. aureus of Malaysian isolate and to evaluate the clinical responses such as temperature, respiratory rates and heart rates in vaccinated cows. S. aureus is a major causative bacteria in clinical and subclinical types of mastitis in cows. Four concentrations of the bacterin (10(6), 10(7), 10(8) and 10(9) cfu/ml of the local isolate of S. aureus) were prepared using Aluminium potassium sulfate adjuvant. Thirty cows were grouped into four treatment groups (B, C, D and E) with a fifth group as control (A). These groups were vaccinated intramuscularly(IM) with the prepared mono- valent vaccine and its influence on the vital signs were intermittently measured. The mean of rectal temperature was significantly different (p(<) 0.05) at Ohr Post Vaccination [1]" in groups D and E (39.5 +/- 0.15 degrees C and 39.4 +/- 0.15 degrees C respectively) and at 3 h PV in groups C, D and E (39.8 +/- 0.14 degrees C, 39.9 +/- 0.14 degrees C and 40.3 +/- 0.14 degrees C respectively) compared to the control group. This indicated a sharp increased rectal temperatures between Ohr and 3 h PV in groups C, D and E which later declined at 24 h PV. The mean of rectal temperature of group E was significantly different (p(<) 0.05) at weeks 1 and 2 PV (39.87 +/- 0.19 degrees C and 39.80 +/- 0.18 degrees C respectively) compared to the control group. The mean of heart rate was significantly different (p(<) 0.05) at week 1 PV in groups D and E (83.0 +/- 3.8 beats/minute and 80.0 +/- 3.8 degrees C respectively) compared to control. A trending decrease was however observed in heart rates of group E from weeks through 4 PV and in group D from weeks 1 through 3 PV. The mean of respiratory rates was significantly different (p(<) 0.05) at week 3 PV in group B and D (31.0 +/- 1.2 breaths/minute and 28.0 +/- 1.2 breaths/minute) compared to control. In conclusion, this study highlights responses of these vital signs due to vaccination against S. aureus causing mastitis in cows. To the best of our knowledge the findings of this study adds value to the shallow literature on vital signs alterations in cows vaccinated against mastitis as elevated levels of temperature and heart rates of group D and E indicated obvious response.
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页码:101 / 105
页数:5
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