Optimization of L-Asparaginase Production by Isolated Aspergillus niger using Sesame Cake in a Column Bioreactor

Acute lymphocytic leukemia is a common leukemia characterized by frequent infections and anemia Thousands of new cases are diagnosed each year worldwide L-Asparaginase (E C 3 5 1 1), also known as L-asparagine amino hydrolase, is a potential antitumor enzyme that catalyses the hydrolysis of L-asparagine into L-aspartic acid and ammonia L-Asparaginase production was investigated in the isolated filamentous fungi on sesame cake using solid state fermentation (SSF) L-Asparaginase production using isolated Aspergillus niger grown on sesame cake has been optimized in a column bioreactor using a statistically-based method The 23 BHH design having eight different fermentation conditions was applied to evaluate their significance on L-Asparaginase activity, where the three independent variables evaluated were Aeration, Thickness of Solid Substrate Bed and Fermentation Temperature Aeration and Temperature were identified to be important variables and had a positive effect on responses, however, the thickness of solid substrate had an insignificant effect on L-Asparaginase activity within the tested range The optimum fermentation condition for L-Asparaginase activity consisted of high aeration (0 4 vvm), deeper thickness of bed (22 cm) and high fermentation temperature (32 degrees C), respectively Under optimum levels, a maximum L Asparaginase activity of 344 2132 IU was predicted which is slightly less than the activity at batch level