Development and evaluation of consolidated bioprocessing yeast for ethanol production from ionic liquid-pretreated bagasse

被引：22

作者：

Amoah, Jerome ^{[1
]}

Ishizue, Naoya ^{[2
]}

Ishizaki, Miki ^{[2
]}

Yasuda, Misa ^{[2
]}

Takahashi, Kenji ^{[3
]}

Ninomiya, Kazuaki ^{[4
]}

Yamada, Ryosuke ^{[5
]}

Kondo, Akihiko ^{[1
,2
]}

Ogino, Chiaki ^{[2
]}

机构：

[1] Kobe Univ, Grad Sch Sci Technol & Innovat, Nada Ku, 1-1 Rokkodai, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Grad Sch Engn, Dept Chem Sci & Engn, Nada Ku, 1-1 Rokkodai, Kobe, Hyogo 6578501, Japan

[3] Kanazawa Univ, Grad Sch Nat Sci & Technol, Div Mat Engn, Kanazawa, Ishikawa 9201192, Japan

[4] Kanazawa Univ, Inst Nat & Environm Technol, Kanazawa, Ishikawa 9201192, Japan

[5] Osaka Prefecture Univ, Dept Chem Engn, Naka Ku, 1-1 Gakuencho, Sakai, Osaka 5998531, Japan

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 245卷

关键词：

Bioethanol; Lignocellulose; Cocktail delta-integration; Cell surface display; Enzyme activity; LIGNOCELLULOSIC MATERIALS; SACCHAROMYCES-CEREVISIAE; ENZYMATIC-HYDROLYSIS; AMORPHOUS CELLULOSE; FERMENTATION; BIOMASS; SACCHARIFICATION; BIOETHANOL; TECHNOLOGIES; BIOFUEL;

D O I：

10.1016/j.biortech.2017.05.171

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

This work aimed to study the use of consolidated bioprocess (CBP) yeast expressing five cellulase genes (BGL, XYNII, EGII, CBHI and CBHII) for ethanol production from ionic liquid-pretreated bagasse and Laubholz unbleached Kraft pulp (LUKP). A proposed screening method shows that the optimal cellulase ratio varies for each biomass substrate, and thus it is essential to breed CBP yeast having optimal cellulase-displaying ratio for the target biomass. CBP yeast specialized towards bagasse produced 0.93 g/l ethanol whiles that for LUKP produced 0.71 g/l ethanol, which is approximately 4 and 2-fold, respectively, higher than that of the wild type. The cell-surface displayed enzymes synergistically contributed to the degradation of the biomass. The developed CBP yeast is a potential cheap source for consolidated bioprocessing of ethanol and the proposed screening method can be used for matching CBP yeast to a target biomass. (C) 2017 Published by Elsevier Ltd.

引用

页码：1413 / 1420

页数：8

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