One-step method embedding superoxide dismutase and gold nanoparticles in silica sol-gel network in the presence of cysteine for construction of third-generation biosensor

被引：58

作者：

Di, Junwei ^{[1
]}

Peng, Shaohua ^{[1
]}

Shen, Chunping ^{[1
]}

Gao, Yansheng ^{[1
]}

Tu, Yifeng ^{[1
]}

机构：

[1] Suzhou Univ, Dept Chem, Suzhou 215006, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 23卷 / 01期

基金：

中国国家自然科学基金;

关键词：

superoxide dismutase; direct electron transfer; gold nanoparticles; sol-gel; self-assembly;

D O I：

10.1016/j.bios.2007.03.011

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A new and one-step method has been developed for the fabrication of superoxide dismutase (SOD) based biosensor. This method was used to form a silica sol-gel (SG) thin film and to immobilize SOD and gold nanoparticles (GNPs) in silica SG network for the fabrication of biosensor. The immobilized superoxide dismutase realized direct electron transfer between the enzyme and electrode surface, and the rate constants of the electrochemical process (k(s)) of SOD was markedly enhanced by GNPs. The electrochemical performance and influencing factors of the resulting biosensor were studied in detail. The resulting biosensor exhibited fast amperometric response to superoxide anion. The calibration range of superoxide anion was from 0.05 to 0.4 mu mol L-1. The proposed method exhibited the benefits of the advantages of self-assembly, nanoparticles and SG techniques. The fabrication of the SOD-modified electrode was easy and simple. The biosensor exhibited high sensitivity and long-term stability. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：88 / 94

页数：7

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