Trehalose-6-phosphate synthase 1 is not the only active TPS in Arabidopsis thaliana

被引：67

作者：

Delorge, Ines ^{[1
,2
]}

Figueroa, Carlos M. ^{[3
]}

Feil, Regina ^{[3
]}

Lunn, John E. ^{[3
]}

Van Dijck, Patrick ^{[1
,2
]}

机构：

[1] Katholieke Univ Leuven, VIB, Dept Mol Microbiol, B-3001 Leuven, Belgium

[2] Katholieke Univ Leuven, Mol Cell Biol Lab, B-3001 Leuven, Belgium

[3] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany

来源：

BIOCHEMICAL JOURNAL | 2015年 / 466卷

关键词：

Arabidopsis thaliana; Saccharomyces cerevisiae; trehalose; trehalose phosphatase (TPP); 6-phosphate; trehalose-6-phosphate synthase (TPS); ESCHERICHIA-COLI-OTSA; SACCHAROMYCES-CEREVISIAE; TREHALOSE; 6-PHOSPHATE; SELAGINELLA-LEPIDOPHYLLA; VEGETATIVE GROWTH; PROTEIN-KINASE; YEAST; EXPRESSION; MUTANT; GENE;

D O I：

10.1042/BJ20141322

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trehalose metabolism is essential for normal growth and development in higher plants. It is synthesized in a two-step pathway catalysed by TPS (trehalose-6-phosphate synthase) and trehalose phosphatase. Arabidopsis thaliana has 11 TPS or TPS-like proteins, which belong to two distinct clades: class I (AtTPS1-AtTPS4) and class II (AtTPS5-AtTPS11). Only AtTPS1 has previously been shown to have TPS activity. A. thaliana tps1 Delta mutants fail to complete embryogenesis and rescued lines have stunted growth and delayed flowering, indicating that AtTPS1 is important throughout the life cycle. In the present study, we show that expression of AtTPS2 or AtTPS4 enables the yeast tps1 Delta tps2 Delta mutant to grow on glucose and accumulate Tre6P (trehalose 6-phosphate) and trehalose. Class II TPS genes did not complement the yeastmutant. Thus A. thaliana has at least three catalytically active TPS isoforms, suggesting that loss of Tre6P production might not be the only reason for the growth defects of A. thaliana tps1 mutants.

引用

页码：283 / 290

页数：8

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