Effect of the degree of substitution of cyclodextrin derivatives on chiral separations by high-performance liquid chromatography and capillary electrophoresis

被引:68
作者
Szeman, J [1 ]
Ganzler, K [1 ]
Salgo, A [1 ]
Szejtli, J [1 ]
机构
[1] TECH UNIV BUDAPEST,DEPT BIOCHEM & FOOD TECHNOL,H-1111 BUDAPEST,HUNGARY
关键词
mobile phase composition; buffer composition; enantiomer separation; cyclodextrins;
D O I
10.1016/0021-9673(95)01312-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Optical isomers of some basic racemic drugs (oxprenolol, AMEBD, ephedrine) were separated by highperformance liquid chromatography (HPLC) and/or capillary electrophoresis (CE) using carboxymethyl-P-cyclo dextrin (CMBCD) with various degree of substitution (DS). The effects of the separation conditions (pH, concentration and DS of CMBCD) were studied and compared using CE and HPLC. The degree of substitution had a significant effect on the resolution of the optical isomers and the ionic strength of the separation media, hence the use of well characterized CD derivatives is crucial. Different optimum DS values for the same test samples were obtained when HPLC or CE was used.
引用
收藏
页码:423 / 431
页数:9
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