Electrochemical Sensor for Detection of Mifepristone and Cinnamic acid for Treatment of Uterine Fibroids using Immobilized Double-Stranded DNA on the AuNPs modified glassy carbon electrode

This research was done to create immobilized dsDNA on Au/GCE for an electrochemical sensor of mifepristone and cinnamic acid (a component of guizhi fuling) as uterine fibroids medications. Deposition of Au nanoparticles (Au NPs) on GCE and immobilization of dsDNA on Au/GCE were accomplished using the electrodeposition approach. SEM and XRD structural investigations of electrodeposited Au NPs revealed that they were electrodeposited in a spherical shape with a fcc crystal structure. The CV and DPV electrochemical measurements indicated that Au NPs grew successfully on the GCE surface and that dsDNA was immobilized on Au/GCE in a stable manner. Further electrochemical investigations using DPV and ampermetery revealed that the simultaneous detection of mifepristone and cinnamic acid on dsDNA on Au/GCE was stable, selective, and sensitive. The linear range, detection limit, and sensitivity for determining mifepristone were 7.5 ng/ml and 0.40031 ??A/mg ml-1 and 0-120 mg/ml, respectively, and for determining cinnamic acid, the linear range, detection limit, and sensitivity were 2.1 ng/ml and 1.42117 ??A/mg ml-1 and 0-41.25 mg/ml, respectively. The practical capability and accuracy of dsDNA/Au/GCE were used for amperomtric determination of mifepristone and cinnamic acid in Mifeprex and Gui Zhi Fu Ling tablets, which indicated a high level of precision for practical ability in medicine sample analysis utilizing dsDNA/Au/GCE.