Identification of differentially expressed thyroid hormone responsive genes from the brain of the Mexican Axolotl (Ambystoma mexicanum)

被引:29
作者
Huggins, P. [2 ]
Johnson, C. K. [1 ]
Schoergendorfer, A. [2 ]
Putta, S. [1 ]
Bathke, A. C. [2 ]
Stromberg, A. J. [2 ]
Voss, S. R. [1 ]
机构
[1] Univ Kentucky, Dept Biol, Lexington, KY 40506 USA
[2] Univ Kentucky, Dept Stat, Lexington, KY 40506 USA
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2012年 / 155卷 / 01期
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
Metamorphosis; Salamander; Microarray; INDUCED METAMORPHOSIS; TRANSCRIPTIONAL RESPONSE; MICROARRAY ANALYSIS; SALAMANDER; RAT; PITUITARY; RECEPTOR; MODEL; MECHANISMS; RESOURCES;
D O I
10.1016/j.cbpc.2011.03.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Mexican axolotl (Ambystoma mexicanum) presents an excellent model to investigate mechanisms of brain development that are conserved among vertebrates. In particular, metamorphic changes of the brain can be induced in free-living aquatic juveniles and adults by simply adding thyroid hormone (T4) to rearing water. Whole brains were sampled from juvenile A. mexicanum that were exposed to 0, 8, and 18 days of 50 nM T4, and these were used to isolate RNA and make normalized cDNA libraries for 454 DNA sequencing. A total of 1,875,732 high quality cDNA reads were assembled with existing ESTs to obtain 5884 new contigs for human RefSeq protein models, and to develop a custom Affymetrix gene expression array (Amby_002) with approximately 20,000 probe sets. The Amby_002 array was used to identify 303 transcripts that differed statistically (p<0.05, fold change >1.5) as a function of days of T4 treatment. Further statistical analyses showed that Amby_002 performed concordantly in comparison to an existing, small format expression array. This study introduces a new A. mexicanum microarray resource for the community and the first lists of T4-responsive genes from the brain of a salamander amphibian. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:128 / 135
页数:8
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