The Influence of Hypoxia and Fibrinogen Variants on the Expansion and Differentiation of Adipose Tissue-Derived Mesenchymal Stem Cells

被引：41
作者：
Weijers, Ester M. [1 ]
Van den Broek, Lenie J. [2 ]
Waaijman, Taco [2 ]
Van Hinsbergh, Victor W. M. [1 ]
Gibbs, Susan [2 ]
Koolwijk, Pieter [1 ]
机构：
[1] Vrije Univ Amsterdam Med Ctr, Dept Physiol, Inst Cardiovasc Res ICaR VU, NL-1081 BT Amsterdam, Netherlands
[2] Vrije Univ Amsterdam Med Ctr, Dept Dermatol, Inst Cardiovasc Res ICaR VU, NL-1081 BT Amsterdam, Netherlands
来源：
TISSUE ENGINEERING PART A | 2011年 / 17卷 / 21-22期
关键词：
REDUCED OXYGEN-TENSION; STROMAL CELLS; LIFE-SPAN; IN-VITRO; CHONDROGENESIS; PROLIFERATION; FIBROBLASTS; EXPRESSION; CULTURE; MARKERS;
D O I：
10.1089/ten.tea.2010.0661
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Upon implantation of tissue-engineered scaffolds, hypoxia will occur until neovascularization takes place. In vivo, the temporary fibrin matrix forms a suitable matrix for this process and fibrin variants can influence the extent of neovascularization. In this study, the influence of oxygen tension and naturally occurring fibrinogen variants on adipose tissue-derived mesenchymal stem cell (ASC) expansion and differentiation were determined. ASC proliferated 1.7-fold faster in 1% oxygen and showed reduced cell aging, and their stemness was preserved. The stem cell surface marker expression was similar in 1% and 20% oxygen. The various fibrinogen coatings did not influence ASC expansion and differentiation. Differentiation of ASC toward adipogenic and osteogenic lineages was improved in 20% oxygen, whereas 1% oxygen improved chondrogenic differentiation. In conclusion, optimal oxygen concentrations vary for the intended ASC application, and fibrinogen variants, which can be used to influence neovascularization, do not alter ASC behavior. These data emphasize the importance of oxygen concentrations during stem cell growth and differentiation.
引用
收藏
页码：2675 / 2685
页数：11
相关论文
共 35 条
[1]   Optimization of mesenchymal stem cell expansion procedures by cell separation and culture conditions modification [J].
Carrancio, Soraya ;
Lopez-Holgado, Natalia ;
Sanchez-Guijo, Fermin M. ;
Villaron, Eva ;
Barbado, Victoria ;
Tabera, Soraya ;
Diez-Campelo, Maria ;
Blanco, Juan ;
San Miguel, Jesuis F. ;
Consuelo del Canizo, M. .
EXPERIMENTAL HEMATOLOGY, 2008, 36 (08) :1014-1021
[2]   Telomere measurement by quantitative PCR [J].
Cawthon, RM .
NUCLEIC ACIDS RESEARCH, 2002, 30 (10) :e47
[3]   Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1 [J].
Ceradini, DJ ;
Kulkarni, AR ;
Callaghan, MJ ;
Tepper, OM ;
Bastidas, N ;
Kleinman, ME ;
Capla, JM ;
Galiano, RD ;
Levine, JP ;
Gurtner, GC .
NATURE MEDICINE, 2004, 10 (08) :858-864
[4]   Hypoxic preconditioning induces the expression of prosurvival and proangiogenic markers in mesenchymal stem cells [J].
Chacko, Simi M. ;
Ahmed, Shabnam ;
Selvendiran, Karuppaiyah ;
Kuppusamy, M. Lakshmi ;
Khan, Mahmood ;
Kuppusamy, Periannan .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2010, 299 (06) :C1562-C1570
[5]   Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].
Dominici, M. ;
Le Blanc, K. ;
Mueller, I. ;
Slaper-Cortenbach, I. ;
Marini, F. C. ;
Krause, D. S. ;
Deans, R. J. ;
Keating, A. ;
Prockop, D. J. ;
Horwitz, E. M. .
CYTOTHERAPY, 2006, 8 (04) :315-317
[6]   Angiogenic properties of aged adipose derived mesenchymal stem cells after hypoxic conditioning [J].
Efimenko, Anastasia ;
Starostina, Ekaterina ;
Kalinina, Natalia ;
Stolzing, Alexandra .
JOURNAL OF TRANSLATIONAL MEDICINE, 2011, 9
[7]   Low O2 tensions and the prevention of differentiation of hES cells [J].
Ezashi, T ;
Das, P ;
Roberts, RM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (13) :4783-4788
[8]   Reduced oxygen tension attenuates differentiation capacity of human mesenchymal stem cells and prolongs their lifespan [J].
Fehrer, Christine ;
Brunauer, Regina ;
Laschober, Gerhard ;
Unterluggauer, Hermann ;
Reitinger, Stephan ;
Kloss, Frank ;
Guelly, Christian ;
Gassner, Robert ;
Lepperdinger, Guenter .
AGING CELL, 2007, 6 (06) :745-757
[9]   Instability of standard PCR reference genes in adipose-derived stem cells during propagation, differentiation and hypoxic exposure [J].
Fink, Trine ;
Lund, Pia ;
Pilgaard, Linda ;
Rasmussen, Jeppe Grondahl ;
Duroux, Meg ;
Zachar, Vladimir .
BMC MOLECULAR BIOLOGY, 2008, 9
[10]   Adipose-derived adult stem cells: isolation, characterization, and differentiation potential [J].
Gimble, JM ;
Guilak, F .
CYTOTHERAPY, 2003, 5 (05) :362-369
1234