Determination of xylooligosaccharides produced from enzymatic hydrolysis of beechwood xylan using high-performance anion-exchange chromatography tandem mass spectrometry

被引:11
作者
Rodriguez, Estrella Sanz [1 ,2 ]
Diaz-Arenas, Gloria Lizeth [3 ,4 ,5 ]
Makart, Stefan [6 ]
Ghosh, Debjani [3 ,7 ]
Patti, Antonio Frank [3 ,7 ]
Garnier, Gil [3 ,4 ,5 ]
Tanner, Joanne [3 ,4 ,5 ]
Paull, Brett [1 ,2 ]
机构
[1] Univ Tasmania, Sch Nat Sci, Australian Ctr Res Separat Sci ACROSS, GPO Box 252-75, Hobart, Tas 7001, Australia
[2] Univ Tasmania, Sch Nat Sci, ARC Ind Transformat Res Hub Proc Adv Lignocellulo, Hobart, Tas 7001, Australia
[3] Monash Univ, Dept Chem & Biol Engn, ARC Ind Transformat Res Hub Proc Adv Lignocellulo, Clayton, Vic 3800, Australia
[4] Monash Univ, Bioresource Proc Res Inst Australia BioPRIA, Dept Chem & Biol Engn, Clayton, Vic 3800, Australia
[5] Monash Univ, Dept Chem & Biol Engn, Clayton, Vic, Australia
[6] Thermo Fisher Sci, 5 Caribbean Dr, Scoresby, Vic 3179, Australia
[7] Monash Univ, Sch Chem, Clayton, Vic 3800, Australia
关键词
High-performance anion-exchange; chromatography; Mass spectrometry; Xylooligosaccharides; Beechwood xylan; STRUCTURAL-CHARACTERIZATION; OLIGOSACCHARIDES; MS; BIOMASS; AUTOHYDROLYSIS; FRAGMENTATION; PRETREATMENT; VALIDATION; SEPARATION;
D O I
10.1016/j.chroma.2022.462836
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
High-performance anion-exchange chromatography (HPAEC) coupled with triple quadrupole mass spectrometry (HPAEC-QqQ-MS) was applied to the determination of xylooligosaccharides (XOS) derived from enzymatically hydrolysed commercial xylan from beechwood and the analytical performance and advantages of the method explored. Separation, eluent suppression, electrospray ionisation, and detection options to enhance XOS sensitivity and selectivity were evaluated, delivering a new simple, fast, selective, and sensitive solution for the characterisation of these complex compounds. The method was fully validated in terms of its analytical performance for those XOS for which standards were available, i.e., degree of polymerisation from 1 to 6. The new method was applied to the analysis of xylan hydrolysates obtained by different enzymatic hydrolysis treatments using endo-xylanase from Thermomyces lanuginosus, characterising 25 different XOS and demonstrating the method's utility for future tailoring of enzymatic hydrolysis conditions to obtain desired XOS profiles in such hydrolysates. Linear XOS and 4-O-methyl glucuronic acid (MeGluA) branched XOS were detected by direct injection of the xylan hydrolysates after a simple 10-fold sample dilution and filtration. Identification of XOS detected by HPAEC-QqQ-MS was additionally confirmed using high-resolution orbitrap mass spectrometry (HR-orbitrap-MS). Further, an ultra sensitive and-selective method was developed by using selected reaction monitoring acquisition mode (SRM), increasing signal-to noise ratio and decreasing the limits of detection, opening future applications to low concentrated sample analysis. (c) 2022 Elsevier B.V. All rights reserved.
引用
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页数:13
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