A Ten-Year Molecular Survey on Parvoviruses Infecting Carnivores in Bulgaria

被引：29

作者：

Filipov, C. ^{[1
]}

Desario, C. ^{[2
]}

Patouchas, O. ^{[2
]}

Eftimov, P. ^{[3
]}

Gruichev, G. ^{[4
]}

Manov, V. ^{[1
]}

Filipov, G.

Buonavoglia, C. ^{[2
]}

Decaro, N. ^{[2
]}

机构：

[1] Univ Forestry, Fac Vet Med, Sofia, Bulgaria

[2] Univ Bari, Dept Vet Med, Valenzano, Italy

[3] Sofia Univ St Kliment Ohridski, Fac Biol, Sofia, Bulgaria

[4] Univ Forestry, Fac Forestry, Sofia, Bulgaria

来源：

TRANSBOUNDARY AND EMERGING DISEASES | 2016年 / 63卷 / 04期

关键词：

parvoviruses; carnivores; molecular survey; Bulgaria; real-time polymerase chain reaction; minor groove binder probe assays; CANINE-PARVOVIRUS; GENETIC-ANALYSIS; STRAINS; TYPE-2; DOGS; EPIDEMIOLOGY; ENTERITIS; EVOLUTION; WOLVES; ASSAY;

D O I：

10.1111/tbed.12285

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Parvoviruses represent the most important infectious agents that are responsible for severe to fatal disease in carnivores. This study reports the results of a 10-year molecular survey conducted on carnivores in Bulgaria (n=344), including 262 dogs and 19 cats with gastroenteritis, and 57 hunted wild carnivores. Real-time polymerase chain reaction (qPCR), followed by virus characterization by minor groove binder (MGB) probe assays, detected 216 parvovirus positive dogs with a predominance of canine parvovirus type 2a (CPV-2a, 79.17%) over CPV-2b (18.52%) and CPV-2c (2.31%). Rottweilers and German shepherds were the most frequent breeds among CPV-positive pedigree dogs (n=96). Eighteen cats were found to shed parvoviruses in their faeces, with most strains being characterized as FPLV (n=17), although a single specimen tested positive for CPV-2a. Only two wild carnivores were parvovirus positive, a wolf (Canis lupus) and a red fox (Vulpes vulpes), both being infected by CPV-2a strains.

引用

页码：460 / 464

页数：5

共 36 条

[1] Frequent Cross-Species Transmission of Parvoviruses among Diverse Carnivore Hosts [J].