An aptamer-SWNT biosensor for sensitive detection of protein via mediated signal transduction

被引：13

作者：

Guo, Kai

Wang, Ying

Chen, Hui

Ji, Ji

Zhang, Song

Kong, Jilie

Liu, Baohong ^{[1
]}

机构：

[1] Fudan Univ, Key Lab Mol Engn Polymers, Chinese Minist Educ, Dept Chem, Shanghai 200433, Peoples R China

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2011年 / 13卷 / 07期

关键词：

Aptamer; SWNT; Controlled assembly; Electrochemistry; Biosensor; ELECTROCHEMICAL DETECTION; CARBON; THROMBIN; DNA; RECOGNITION; SELECTION;

D O I：

10.1016/j.elecom.2011.04.016

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

An aptamer-SWNT based electrochemical biosensor is developed for sensitive detection of thrombin via mediated signal transduction. To realize this purpose, a dense monolayer of 16-mercaptohexadecanoic acid was modified on the gold electrode. In the presence of thrombin. SWNTs were controllably assembled on this insulating monolayer, which could mediate efficient electron transfer between the electrode and eletroactive species to generate a larger redox current. Through detecting the redox signal mediated by SWNTs, this strategy could present significant signal amplification and a detection limit of 50 pM thrombin was achieved. Such an aptamer-SWNT based biosensor opens a rapid, selective and sensitive route for thrombin detection and offers a promising strategy for specific protein detection. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：707 / 710

页数：4

共 21 条

[1] A novel electrochemical detection method for aptamer biosensors [J].