Modelling of ceramide interactions with porous graphite carbon in non-aqueous liquid chromatography

被引：7
|
作者：
West, C
Cilpa, G
Gaudin, K
Chaminade, P
Lesellier, E
机构：
[1] Univ Paris 11, LETIAM, IUT Orsay, F-91400 Orsay, France
[2] Fac Pharm Chatenay Malabry, Chim Analyt Lab, F-92296 Chatenay Malabry, France
来源：
JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1087卷 / 1-2期
关键词：
non-aqueous reversed-phase liquid chromatography (NARP-LC); linear solvation energy relationship (LSER); ceramides; porous graphitic carbon (PGC);
D O I：
10.1016/j.chroma.2005.03.026
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Interactions of solutes on porous graphitic carbon (PGC) with non-aqueous mobile phases are studied by the linear solvation energy relationship (LSER). Studies have been carried out with eight binary mixtures composed of a weak solvent (acetonitrile or methanol) and a strong solvent (tetrahydrofuran, n-butanol, CH2Cl2, 1,1,2-trichloro-2,2,1-trifluoroethane). The systematic analysis of a set of test compounds was performed for each solvent mixture in isocratic mode (50:50). The results were compared to those obtained on PGC with hydro-organic liquids and supercritical fluids. They were then correlated with the observed retention behaviour of lipid compounds, more particularly ceramides. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：77 / 85
页数：9
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