Structure of a polysaccharide from a Rhizobium species containing 2-deoxy-β-D-arabino-hexuronic acid

被引：56
作者：
Guentas, L
Pheulpin, P
Michaud, P
Heyraud, A
Gey, C
Courtois, B
Courtois, J
机构：
[1] Univ Picardie, Inst Univ Technol, Dept Gen Biol, Lab Polysaccharides Microbiens & Vegetaux, F-80025 Amiens, France
[2] CNRS, Ctr Rech Macromol Vegetales, UPR 5301, F-38041 Grenoble, France
来源：
CARBOHYDRATE RESEARCH | 2001年 / 332卷 / 02期
关键词：
extracellular polysaccharide; Rhizobium sp; structural analysis; 2-deoxy-arabino-D-hexuronic acid;
D O I：
10.1016/S0008-6215(01)00080-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structure of the extracellular polysaccharide (EPS) produced by the Rhizobium sp. B strain isolated from atypical nodules on alfalfa has been determined using a combination of chemical and physical techniques (methylation analysis, high pH-anion exchange chromatography (HPAEC), mass spectrometry and 1-D and 2-D NMR spectroscopy). As opposed to the EPS from other strains of Rhizobium, the EPS from the sp. B strain contains D-Glc together with L-Rha and 2-deoxy-D-arabino -hexuronic acid. It is a polymer of a repeating unit having the following structure: --> 4)-beta -D-Glcp -(1 --> 4)-alpha -L-Rhap-(1 --> 3)-beta -D-Glcp -(1 --> 4)-2-deoxy-beta -D-GlcpA-(l --> The polysaccharide also contains 0.6 O-acetyl groups per sugar which have not been located. (C) 2001 Elsevier Science Ltd. All rights reserved.
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页码：167 / 173
页数：7
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