Characterization and Strain Improvement of a Hypercellulytic Variant, Trichoderma reesei SN1, by Genetic Engineering for Optimized Cellulase Production in Biomass Conversion Improvement

被引：29

作者：

Qian, Yuanchao ^{[1
]}

Zhong, Lixia ^{[2
]}

Hou, Yunhua ^{[3
]}

Qu, Yinbo ^{[1
]}

Zhong, Yaohua ^{[1
]}

机构：

[1] Shandong Univ, Sch Life Sci, State Key Lab Microbial Technol, Jinan, Peoples R China

[2] Shandong Inst Food & Drug Control, Jinan, Peoples R China

[3] Qilu Univ Technol, Bioengn Inst, Jinan, Peoples R China

来源：

FRONTIERS IN MICROBIOLOGY | 2016年 / 7卷

基金：

中国国家自然科学基金;

关键词：

Trichoderma reesei; uracil auxotrophy; beta-glucosidase; biomass conversion; pretreated corncob residues; SOLID-SUBSTRATE FERMENTATION; BETA-GLUCOSIDASE I; HYPOCREA-JECORINA; TRANSFORMATION SYSTEM; ENZYME-SYSTEM; HYDROLYSIS; EXPRESSION; XYLANASE; BAGASSE; VIRIDE;

D O I：

10.3389/fmicb.2016.01349

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The filamentous fungus Trichoderma reesei is a widely used strain for cellulolytic enzyme production. A hypercellulolytic T. reesei variant SN1 was identified in this study and found to be different from the well-known cellulase producers QM9414 and RUT-C30. The cellulose-degrading enzymes of T reesei SN1 show higher endoglucanase (EG) activity but lower beta-glucosidase (BGL) activity than those of the others. A uracil auxotroph strain, SP4, was constructed by pyr4 deletion in SN1 to improve transformation efficiency. The BGL1-encoding gene bgl1 under the control of a modified cbh1 promoter was overexpressed in SP4. A transformant, SPB2, with four additional copies of bgl1 exhibited a 17.1-fold increase in BGL activity and a 30.0% increase in filter paper activity. Saccharification of corncob residues with crude enzyme showed that the glucose yield of SPB2 is 65.0% higher than that of SP4. These results reveal the feasibility of strain improvement through the development of an efficient genetic transformation platform to construct a balanced cellulase system for biomass conversion.

引用

页数：11

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