Characterization and Strain Improvement of a Hypercellulytic Variant, Trichoderma reesei SN1, by Genetic Engineering for Optimized Cellulase Production in Biomass Conversion Improvement

被引：29
作者：
Qian, Yuanchao [1 ]
Zhong, Lixia [2 ]
Hou, Yunhua [3 ]
Qu, Yinbo [1 ]
Zhong, Yaohua [1 ]
机构：
[1] Shandong Univ, Sch Life Sci, State Key Lab Microbial Technol, Jinan, Peoples R China
[2] Shandong Inst Food & Drug Control, Jinan, Peoples R China
[3] Qilu Univ Technol, Bioengn Inst, Jinan, Peoples R China
来源：
FRONTIERS IN MICROBIOLOGY | 2016年 / 7卷
基金：
中国国家自然科学基金;
关键词：
Trichoderma reesei; uracil auxotrophy; beta-glucosidase; biomass conversion; pretreated corncob residues; SOLID-SUBSTRATE FERMENTATION; BETA-GLUCOSIDASE I; HYPOCREA-JECORINA; TRANSFORMATION SYSTEM; ENZYME-SYSTEM; HYDROLYSIS; EXPRESSION; XYLANASE; BAGASSE; VIRIDE;
D O I：
10.3389/fmicb.2016.01349
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The filamentous fungus Trichoderma reesei is a widely used strain for cellulolytic enzyme production. A hypercellulolytic T. reesei variant SN1 was identified in this study and found to be different from the well-known cellulase producers QM9414 and RUT-C30. The cellulose-degrading enzymes of T reesei SN1 show higher endoglucanase (EG) activity but lower beta-glucosidase (BGL) activity than those of the others. A uracil auxotroph strain, SP4, was constructed by pyr4 deletion in SN1 to improve transformation efficiency. The BGL1-encoding gene bgl1 under the control of a modified cbh1 promoter was overexpressed in SP4. A transformant, SPB2, with four additional copies of bgl1 exhibited a 17.1-fold increase in BGL activity and a 30.0% increase in filter paper activity. Saccharification of corncob residues with crude enzyme showed that the glucose yield of SPB2 is 65.0% higher than that of SP4. These results reveal the feasibility of strain improvement through the development of an efficient genetic transformation platform to construct a balanced cellulase system for biomass conversion.
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页数：11
相关论文
共 48 条
[1]   Process technological effects of deletion and amplification of hydrophobins I and II in transformants of Trichoderma reesei [J].
Bailey, MJ ;
Askolin, S ;
Hörhammer, N ;
Tenkanen, M ;
Linder, M ;
Penttilä, M ;
Nakari-Setälä, T .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 58 (06) :721-727
[2]   Enzymatic hydrolysis of maize straw polysaccharides for the production of reducing sugars [J].
Chen, Ming ;
Zhao, Jing ;
Xia, Liming .
CARBOHYDRATE POLYMERS, 2008, 71 (03) :411-415
[3]   Isolation of cellulolytic bacteria from the intestine of Diatraea saccharalis larvae and evaluation of their capacity to degrade sugarcane biomass [J].
Dantur, Karina I. ;
Enrique, Ramon ;
Welin, Bjoern ;
Castagnaro, Atilio P. .
AMB EXPRESS, 2015, 5
[4]   CELLULASE PRODUCTION BY MIXED FUNGI IN SOLID-SUBSTRATE FERMENTATION OF BAGASSE [J].
DUENAS, R ;
TENGERDY, RP ;
GUTIERREZCORREA, M .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1995, 11 (03) :333-337
[5]   MEASUREMENT OF CELLULASE ACTIVITIES [J].
GHOSE, TK .
PURE AND APPLIED CHEMISTRY, 1987, 59 (02) :257-268
[6]  
GOMES I, 1992, APPL MICROBIOL BIOT, V36, P701, DOI 10.1007/BF00183253
[7]   THE DEVELOPMENT OF A HETEROLOGOUS TRANSFORMATION SYSTEM FOR THE CELLULOLYTIC FUNGUS TRICHODERMA-REESEI BASED ON A PYRG-NEGATIVE MUTANT STRAIN [J].
GRUBER, F ;
VISSER, J ;
KUBICEK, CP ;
DEGRAAFF, LH .
CURRENT GENETICS, 1990, 18 (01) :71-76
[8]   Effect of endoglucanases and hemicellulases in magnetic and flotation deinking of xerographic and laser-printed papers [J].
Gübitz, GM ;
Mansfield, SD ;
Böhm, D ;
Saddler, JN .
JOURNAL OF BIOTECHNOLOGY, 1998, 65 (2-3) :209-215
[9]   Cellulases and hemicellulases in the 21st century race for cellulosic ethanol [J].
Gusakov, Alexander V. .
BIOFUELS-UK, 2013, 4 (06) :567-569
[10]   Mixed culture solid substrate fermentation of Trichoderma reesei with Aspergillus niger on sugar cane bagasse [J].
Gutierrez-Correa, M ;
Portal, L ;
Moreno, P ;
Tengerdy, RP .
BIORESOURCE TECHNOLOGY, 1999, 68 (02) :173-178
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