Characterisation of oligomeric and polymeric procyanidins from grape seeds by liquid secondary ion mass spectrometry

被引：3

作者：

de Freitas, VAP

Glories, Y

Bourgeois, G

Vitry, C

机构：

[1] Univ Porto, Fac Ciencias, Dept Quim, P-4150 Porto, Portugal

[2] Univ Bordeaux 2, Fac Oenol, F-33405 Talence, France

[3] Univ Bordeaux 1, CNRS, URA 35, F-33405 Talence, France

来源：

PHYTOCHEMISTRY | 1998年 / 49卷 / 05期

关键词：

Vitis vinifera; Vitaceae; grape seeds; proanthocyanidins; condensed tannins; liquid secondary ionisation mass spectrometry;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several fractions of oligomeric and polymeric procyanidins have been obtained from grape seeds by gel chromatography on Fractogel TSK. Their molecular weights (Mw) were directly determined by Liquid Secondary Ion Mass Spectrometry (LSIMS). We observed that tannins were molecules with a wide range of Mw: from 290 to 3100 (decamers). Catechins (Mw 290), procyanidin dimers (Mw 578), (-)-epicatechin gallate (Mw 442), procyanidin dimer gallates (Mw 730), procyanidin dimer digallates (Mw 882), procyanidin trimers (Mw 866) and procyanidin trimer gallates (Mw 1018), were identified as (M-H)- quasimolecular ions by negative LSIMS. Some structures were elucidated by partial thiolysis and enzymatic hydrolysis. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：1435 / 1441

页数：7

共 28 条

[1] GALLOYLATED PROCYANIDINS IN VITIS-VINIFERA VINE-SHOOTS - SEPARATION AND IDENTIFICATION, USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND GAS-CHROMATOGRAPHY [J].