Rapid and Sensitive Detection of Bovine Coronavirus and Group A Bovine Rotavirus from Fecal Samples by Using One-Step Duplex RT-PCR Assay

被引：15

作者：

Zhu, Wei ^{[1
]}

Dong, Jianbao ^{[1
]}

Haga, Takeshi ^{[1
]}

Goto, Yoshitaka ^{[1
]}

Sueyoshi, Masuo ^{[2
]}

机构：

[1] Miyazaki Univ, Dept Vet Microbiol, Miyazaki 8892192, Japan

[2] Miyazaki Univ, Dept Vet Hyg, Miyazaki 8892192, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2011年 / 73卷 / 04期

关键词：

BCoV; detection; differentiation; group A BRV; one-step duplex RT-PCR; WINTER DYSENTERY; CALF DIARRHEA; NESTED PCR; VIRUS; DIAGNOSIS; ENTERO;

D O I：

10.1292/jvms.10-0401

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Bovine coronavirus (BCoV) and group A bovine rotavirus (BRV) are two of major causes for neonatal calf diarrhea. In the present study, a one-step duplex RT-PCR was established to detect and differentiate BCoV and group A BRV from fecal samples. The sensitivity of this method for BCoV and group A BRV was 10 PFU/100 mu l and I PFU/100 mu l, respectively. Twenty-eight diarrhea fecal samples were detected with this method, the result showed that 2 samples were identified as co-infected with BCoV and group A BRV, 26 samples were group A BRV positive, and 2 samples were negative. It proved that this method is sensitive for clinical fecal samples and is worth applying to laboratory diagnosis for BCoV and group A BRV.

引用

页码：531 / 534

页数：4

共 19 条

[1] PROPAGATION OF THE KAKEGAWA STRAIN OF BOVINE CORONAVIRUS IN SUCKLING MICE, RATS AND HAMSTERS
AKASHI, H
INABA, Y
MIURA, Y
SATO, K
TOKUHISA, S
ASAGI, M
HAYASHI, Y
[J]. ARCHIVES OF VIROLOGY, 1981, 67 (04) : 367 - 370
[2] ATHANASSIOUS R, 1994, CAN VET J, V35, P163
[3] BRIDGER JC, 1987, CIBA F SYMP, V128, P5
[4] Casas I, 1999, J MED VIROL, V57, P145, DOI 10.1002/(SICI)1096-9071(199902)57:2<145::AID-JMV10>3.0.CO
[5] 2-N
[6] Cross-protection studies between respiratory and calf diarrhea and winter dysentery coronavirus strains in calves and RT-PCR and nested PCR for their detection
Cho, KO
Hasoksuz, M
Nielsen, PR
Chang, KO
Lathrop, S
Saif, LJ
[J]. ARCHIVES OF VIROLOGY, 2001, 146 (12) : 2401 - 2419
[7] Nested reverse transcriptase-polymerase chain reaction for the detection of group A rotaviruses
Elschner, M
Prudlo, J
Hotzel, H
Otto, P
Sachse, K
[J]. JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 2002, 49 (02): : 77 - 81
[8] A comparative sequence analysis to revise the current taxonomy of the family Coronaviridae
Gonzaález, JM
Gomez-Puertas, P
Cavanagh, D
Gorbalenya, AE
Enjuanes, L
[J]. ARCHIVES OF VIROLOGY, 2003, 148 (11) : 2207 - 2235
[9] Gröndahl B, 1999, J CLIN MICROBIOL, V37, P1
[10] HOLMES KV, 1996, VIROLOGY, P1075

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