γ9δ2 T-Cell Expansion and Phenotypic Profile Are Reflected in the CDR3δ Repertoire of Healthy Adults

被引：3

作者：

Vyborova, Anna ^{[1
]}

Janssen, Anke ^{[1
]}

Gatti, Lucrezia ^{[1
]}

Karaiskaki, Froso ^{[1
]}

Yonika, Austin ^{[1
]}

van Dooremalen, Sanne ^{[1
]}

Sanders, Jasper ^{[1
]}

Beringer, Dennis X. ^{[1
]}

Straetemans, Trudy ^{[1
,2
]}

Sebestyen, Zsolt ^{[1
]}

Kuball, Juergen ^{[1
,2
]}

机构：

[1] Univ Utrecht, Univ Med Ctr UMC Utrecht, Ctr Translat Immunol, Utrecht, Netherlands

[2] Univ Utrecht, Univ Med Ctr UMC Utrecht, Dept Hematol, Utrecht, Netherlands

来源：

FRONTIERS IN IMMUNOLOGY | 2022年 / 13卷

关键词：

human gamma 9 delta 2 T cells; CDR3; delta; adult V delta 2 repertoire; differentiation profile; public clonotypes; NKG2D; CD94/NKG2A(B); RECEPTORS; LYMPHOCYTES; RECOGNITION; EXPRESSION; TCR; ACTIVATION; GENERATION; SELECTION; SUBSETS; REGIONS;

D O I：

10.3389/fimmu.2022.915366

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

gamma 9 delta 2T cells fill a distinct niche in human immunity due to the unique physiology of the phosphoantigen-reactive gamma 9 delta 2TCR. Here, we highlight reproducible TCR delta complementarity-determining region 3 (CDR3 delta) repertoire patterns associated with gamma 9 delta 2T cell proliferation and phenotype, thus providing evidence for the role of the CDR3 delta in modulating in vivo T-cell responses. Features that determine gamma 9 delta 2TCR binding affinity and reactivity to the phosphoantigen-induced ligand in vitro appear to similarly underpin in vivo clonotypic expansion and differentiation. Likewise, we identify a CDR3 delta bias in the gamma 9 delta 2T cell natural killer receptor (NKR) landscape. While expression of the inhibitory receptor CD94/NKG2A is skewed toward cells bearing putative high-affinity TCRs, the activating receptor NKG2D is expressed independently of the phosphoantigen-sensing determinants, suggesting a higher net NKR activating signal in T cells with TCRs of low affinity. This study establishes consistent repertoire-phenotype associations and justifies stratification for the T-cell phenotype in future research on gamma 9 delta 2TCR repertoire dynamics.

引用

页数：13