Visualization of solute migration in chromatographic columns - Influence of the frit porosity

被引:27
作者
Broyles, BS
Shalliker, RA
Guiochon, G [1 ]
机构
[1] Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA
[2] Oak Ridge Natl Lab, Div Chem & Analyt Sci, Oak Ridge, TN 37831 USA
关键词
visualization of solute migration; band profiles; porosity; axial dispersion; frits; iodine;
D O I
10.1016/S0021-9673(01)00623-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The dual-perspective, on-column detection method previously described was used to observe the effects of the inlet frit on the profiles of chromatographic bands. Visualization of bands of iodine was achieved by injecting its dilute solutions in carbon tetrachloride into a glass column packed with a C-18-bonded silica and eluted with carbon tetrachloride, which has the same refractive index as the packing material. The bands were photographed on-column with two standard 35-mm SLR cameras oriented at right angle. The photographs were scanned and the digitized images of the sample bands analyzed with proper software. A number of columns, as similar as possible, were fitted with different 2- and 10-mum porosity stainless steel frits. Subsequent analysis of the digitized band images revealed irregularities in the band shape resulting from frit contributions to band dispersion. The 2-mum frits produced more dramatic effects overall than the coarser frits. Local axial dispersion coefficient values, expressed as local reduced plate height, were calculated. The results demonstrate the possibly damaging effects of the frit on the band shape. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:1 / 22
页数:22
相关论文
共 14 条
[1]   AN NMR RELAXATION STUDY OF AQUEOUS-SOLUTIONS OF GD(III) CHELATES [J].
AIME, S ;
BOTTA, M ;
ERMONDI, G .
JOURNAL OF MAGNETIC RESONANCE, 1991, 92 (03) :572-580
[2]   NMR IMAGING OF THE CHROMATOGRAPHIC PROCESS - DEPOSITION AND REMOVAL OF GADOLINIUM IONS ON A REVERSED-PHASE LIQUID-CHROMATOGRAPHIC COLUMN [J].
BAYER, E ;
BAUMEISTER, E ;
TALLAREK, U ;
ALBERT, K ;
GUIOCHON, G .
JOURNAL OF CHROMATOGRAPHY A, 1995, 704 (01) :37-44
[3]   VISUALIZATION OF CHROMATOGRAPHIC SEPARATIONS BY NMR IMAGING [J].
BAYER, E ;
MULLER, W ;
ILG, M ;
ALBERT, K .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1989, 28 (08) :1029-1032
[4]   Visualization of viscous fingering in chromatographic columns [J].
Broyles, BS ;
Shalliker', RA ;
Cherrak, DE ;
Guiochon, G .
JOURNAL OF CHROMATOGRAPHY A, 1998, 822 (02) :173-187
[5]   COLUMN EFFICIENCY AND RADIAL HOMOGENEITY IN LIQUID-CHROMATOGRAPHY [J].
FARKAS, T ;
CHAMBERS, JQ ;
GUIOCHON, G .
JOURNAL OF CHROMATOGRAPHY A, 1994, 679 (02) :231-245
[6]   GADOLINIUM-DTPA AS A CONTRAST AGENT IN MR IMAGING - THEORETICAL PROJECTIONS AND PRACTICAL OBSERVATIONS [J].
GADIAN, DG ;
PAYNE, JA ;
BRYANT, DJ ;
YOUNG, IR ;
CARR, DH ;
BYDDER, GM .
JOURNAL OF COMPUTER ASSISTED TOMOGRAPHY, 1985, 9 (02) :242-251
[7]   Refining the scale-up of chromatographic separations [J].
Lode, FG ;
Rosenfeld, A ;
Yuan, QS ;
Root, TW ;
Lightfoot, EN .
JOURNAL OF CHROMATOGRAPHY A, 1998, 796 (01) :3-14
[8]  
RUNGE VM, 1989, ENHANCED MAGNETIC RE, pCH5
[9]   Visualization of solute migration in liquid chromatography columns [J].
Shalliker, RA ;
Broyles, BS ;
Guiochon, G .
JOURNAL OF CHROMATOGRAPHY A, 1998, 826 (01) :1-13
[10]   Visualization of sample introduction in liquid chromatographic columns - Contribution of a flow distributor on the sample band shape [J].
Shalliker, RA ;
Broyles, BS ;
Guiochon, G .
JOURNAL OF CHROMATOGRAPHY A, 1999, 865 (1-2) :83-95