The Effect of Interferon-Beta Therapy on T-Helper 17/miR-326 and T-Helper 1/miR-29b-3p Axis in Relapsing-Remitting Multiple Sclerosis Patients

被引:2
作者
Karimi, Leila [1 ]
Eskandari, Nahid [1 ]
Shaygannejad, Vahid [2 ]
机构
[1] Isfahan Univ Med Sci, Sch Med, Dept Immunol, Esfahan, Iran
[2] Isfahan Univ Med Sci, Alzahra Res Inst, Isfahan Neurosci Res Ctr, Esfahan, Iran
关键词
Interferon-beta; Hsa-miR-326; MxA; Hsa-miR-29b-3p; 17; CELLS; EXPRESSION; MICRORNAS; PATHOGENESIS; BIOMARKERS; MIR-326; BLOOD; TH17;
D O I
10.1159/000519777
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: We aimed to evaluate the therapeutic effects of interferon-beta (IFN-beta) on hsa-miR29b-3p and hsa-miR326 in isolated T-helper (Th)1 and Th17 cells expressed by relapsing-remitting multiple sclerosis (RRMS) patients before and after 1 year of treatment with IFN-beta. Methods: The study was done on 19 RRMS patients pre- and posttreatment with IFN-beta to evaluate the frequency of Th1 and Th17 cells by flow cytometry. The expression level of hsa-miR-29b-3p and hsa-miR-326 in isolated Th1 and Th17 cells was assessed by quantitative polymerase chain reaction. Enzyme-linked immunosorbent assay was also used to measure the plasma levels of I interferon -gamma and interleukin (IL)-17A. Results: Th17 cells and plasma levels of IL-17A decreased in RRMS patients after IFN-beta therapy but hsa-miR-29b-3p and hsa-miR-326 expression had no significant change in treated RRMS patients versus baseline. MxA gene expression was significantly induced upon IFN-beta therapy in patients with RRMS. Conclusion: IFN-beta therapy is more effective on Th17 than Th1, but it does not reform altered expression of hsa-miR-326 and hsa-miR-29b-3p in Th17 and Th1, respectively.
引用
收藏
页码:177 / 185
页数:9
相关论文
共 39 条
[1]   Alterations in serum MMP-8, MMP-9, IL-12p40 and IL-23 in multiple sclerosis patients treated with interferon-β1b [J].
Alexander, J. S. ;
Harris, M. K. ;
Wells, S. R. ;
Mills, G. ;
Chalamidas, K. ;
Ganta, V. C. ;
McGee, J. ;
Jennings, M. H. ;
Gonzalez-Toledo, E. ;
Minagar, A. .
MULTIPLE SCLEROSIS JOURNAL, 2010, 16 (07) :801-809
[2]   Interferon-β exacerbates Th17-mediated inflammatory disease [J].
Axtell, Robert C. ;
Raman, Chander ;
Steinman, Lawrence .
TRENDS IN IMMUNOLOGY, 2011, 32 (06) :272-277
[3]   T helper type 1 and 17 cells determine efficacy of interferon-β in multiple sclerosis and experimental encephalomyelitis [J].
Axtell, Robert C. ;
de Jong, Brigit A. ;
Boniface, Katia ;
van der Voort, Laura F. ;
Bhat, Roopa ;
De Sarno, Patrizia ;
Naves, Rodrigo ;
Han, May ;
Zhong, Franklin ;
Castellanos, Jim G. ;
Mair, Robert ;
Christakos, Athena ;
Kolkowitz, Ilan ;
Katz, Liat ;
Killestein, Joep ;
Polman, Chris H. ;
Malefyt, Rene de Waal ;
Steinman, Lawrence ;
Raman, Chander .
NATURE MEDICINE, 2010, 16 (04) :406-U21
[4]   Assessment of Interleukin-17A, Interleukin-10 and Transforming Growth Factor-Beta1 Serum Titers in Relapsing Remitting Multiple Sclerosis Patients Treated with Avonex, Possible Biomarkers for Treatment Response [J].
Balasa, Rodica ;
Maier, Smaranda ;
Voidazan, Septimiu ;
Hutanu, Adina ;
Bajko, Zoltan ;
Motataianu, Anca ;
Tilea, Brandusa ;
Tiu, Cristina .
CNS & NEUROLOGICAL DISORDERS-DRUG TARGETS, 2017, 16 (01) :93-101
[5]   SURFACE EXPRESSION OF ALPHA-4 INTEGRIN BY CD4 T-CELLS IS REQUIRED FOR THEIR ENTRY INTO BRAIN PARENCHYMA [J].
BARON, JL ;
MADRI, JA ;
RUDDLE, NH ;
HASHIM, G ;
JANEWAY, CA .
JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 177 (01) :57-68
[6]   Phenotypical and functional characterization of T helper 17 cells in multiple sclerosis [J].
Brucklacher-Waldert, Verena ;
Sturner, Klarissa ;
Kolster, Manuela ;
Wolthausen, Julia ;
Tolosa, Eva .
BRAIN, 2009, 132 :3329-3341
[7]   Dysregulated MicroRNA involvement in Multiple Sclerosis by induction of T Helper 17 Cell Differentiation [J].
Chen, Chen ;
Zhou, Yifan ;
Wang, Jingqi ;
Yan, Yaping ;
Peng, Lisheng ;
Qiu, Wei .
FRONTIERS IN IMMUNOLOGY, 2018, 9
[8]   The role of microRNAs in the pathogenesis of autoimmune diseases [J].
Chen, Ji-Qing ;
Papp, Gabor ;
Szodoray, Peter ;
Zeher, Margit .
AUTOIMMUNITY REVIEWS, 2016, 15 (12) :1171-1180
[9]   Small non-coding RNA signature in multiple sclerosis patients after treatment with interferon-β [J].
De Felice, Bruna ;
Mondola, Paolo ;
Sasso, Anna ;
Orefice, Giuseppe ;
Bresciamorra, Vincenzo ;
Vacca, Giovanni ;
Biffali, Elio ;
Borra, Marco ;
Pannone, Raimondo .
BMC MEDICAL GENOMICS, 2014, 7
[10]   Altered miRNA expression in T regulatory cells in course of multiple sclerosis [J].
De Santis, Giuseppe ;
Ferracin, Manuela ;
Biondani, Andrea ;
Caniatti, Luisa ;
Tola, Maria Rosaria ;
Castellazzi, Massimiliano ;
Zagatti, Barbara ;
Battistini, Luca ;
Borsellino, Giovanna ;
Fainardi, Enrico ;
Gavioli, Riccardo ;
Negrini, Massimo ;
Furlan, Roberto ;
Granieri, Enrico .
JOURNAL OF NEUROIMMUNOLOGY, 2010, 226 (1-2) :165-171